Buang Abdullah
Indonesian Center for Rice Research, Jl. Raya 9, Sukamandi-Subang 41256 Phone (0260) 520157

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Yield Stability and Adaptability of Aromatic New Plant Type (NPT) Rice Lines Lestari, Angelita Puji; Abdullah, Buang; Junaedi, Ahmad; Aswidinnoor, Hajrial
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 3 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Aromatic new plant type (NPT) rice lines were selected to obtain high yielding and aromatic lines. The objectives of the research were to study the yield stability and adaptability of 35 NPT rice lines across different environment, with Ciherang and Sintanur as check varieties. The lines planted at two locations, Bogor and Pusakanagara in two seasons (2009 dry and wet seasons, DS-WS). The experiment used  randomized complete block design (RCBD), with three replications. The 21-day-old seedlings were planted with spacing of 20 cm x 20 cm, with a plot size of 2 m x 5 m. Yield stability and adaptability were estimated by using coefficient regression (bi) and general mean of yield by Finlay-Wilkinson method. Combined analysis of variance showed that lines (G), environment (E), and the G x E interaction were significantly different. Lines showed different stability and adaptability. Several lines yielded higher than Ciherang variety. Nine lines were classified as stable and widely adapted at the marginal environment, i.e IPB 116-F-3-1, IPB 117-F-4-1, IPB-117-F-14-2, IPB-117-F-15-2, IPB-117-F-17-5, IPB 140-F-1-1, 140-F-IPB, 2-1, IPB 140-F-3, and IPB 149-F-2. Lines IPB 113-F-2, IPB 140-F-4, IPB 140-F-6, IPB 140-F-7, and B11738-MR-Si-1-2-1-2 were not stable and adapted only in optimum environmental condition (bi > 1) while IPB 116-F-46-1, IPB-117-F 17-4, IPB-117-F 18-3 and B11955-MR-84-1-4 has the value of bi < 1 or adaptable to marginal environments.   Keywords:  NPT rice, aromatic rice, yield stability
Anther Culture Ability from Crossess Between Upland and New Plant Types of Rice Safitri, Heni; Purwoko, Bambang Sapta; Wirnas, Desta; Dewi, Iswari S.; Abdullah, Buang
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 2 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Anther culture provides rapid route in obtaining pure lines in a single generation through producing green haploid plants that may be spontaneously doubled. This technique has been used for crop improvement especially in rice. The objective of this research was to determine regeneration ability of eight F1s derived from crossess between upland and new plant types of rice and from their four parents through anther culture. Completely randomized design with 25 replications was used in this research. Treatments consisted of four parent lines/varieties i.e. P1 (Fatmawati and BP360E-MR-79-2), P2 (Fulan Telo Gawa and Fulan Telo Mihat) and eight F1s obtained from reciprocal crosses of P1 and P2. Callus induction medium was based on N6 medium + 2.0 mg L-1 NAA + 0.5 mg L-1  kinetin + 10-3  M Putrescine, while regeneration medium was based on MS + 0.5 mg L-1  NAA + 2.0 mg L-1  kinetin + 10-3  M Putrescine. The result indicated that F1 derived from Fatmawati x Fulan Telo Gawa (5.00% green plants per total anther) and their reciprocal (3.80% green plants per total anther) crosses were the most responsive genotypes in rice anther culture (had high anther culture ability). The F1 genotypes were more effective to produce green and doubled haploid plants in rice anther culture than their parents. From this research, 161 double haploid plants (29.81%) from total acclimated green plantlets were obtained.  Keywords: anther culture, upland rice, new plant type of rice
Pembentukan Galur Haploid Ganda Padi Gogo dengan Sifat-Sifat Tipe Baru melalui Kultur Antera Herawati, Reny; Purwoko, Bambang S.; Khumaida, Nurul; Dewi, Iswari S.; Abdullah, Buang
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 36, No 3 (2008): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The breeding of upland rice with New Plant Type characters in relatively short time can be done by using anther culture technique. The technique has been recognized as a rapid and efficient technology for crop improvement. Plant materials used in this research were F1 crossing P1 (Fatmawati x Way Rarem), P2 (Fatmawati x SGJT-28),  P3 (Fatmawati x SGJT-36), P4  (Way Rarem x Fatmawati), P5 (SGJT-28 x Fatmawati), and P6  (SGJT-36 x Fatmawati). Media for calli induction (N6) and regeneration (MS) were according to Dewi methods (2003). The results of this study indicated that P3 (Fatmawati x SGJT-36) and P6 (SGJT-36 x Fatmawati)  from resiprocal crosses gave better response in anther culture than the others crosses for their calli induction and green plant regeneration. From the six F1 crosses, 348 (53.5%) spontaneous doubled haploid (DH) pure lines were obtained, six lines from Farmawati x Way Rarem, 13 lines from Fatmawati x SGJT-28, 187 lines from Fatmawati x SGJT-36, three lines from Way Rarem x Fatmawati, five lines from SGJT-28 x Fatmawati, and 134 lines from SGJT-36 x Fatmawati.  These lines should be evaluated and characterized for their agronomical and morphological  traits.    Key words: Anther culture, doubled haploid, callus induction, green plant regeneration        
lntrogression of Bacterial Leaf Blight Resistance Gene from Oryza Minuta J.B. Presl. Ex C. B. Presl. into New Rice Type (Oryza sativa L.) Abdullah, Buang; Brar, D. S.; Carpena, A. L.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 29, No 2 (2001): Buletin Agronomi
Publisher : Bogor Agricultural University

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Abstract

F1 Hybrids, backcross progenies, advanced introgression lines (2n=24) and monosomic alien addition lines or MAALs (2n=25) were successfully produced following embryo rescue between an elite new plant type (NPT) breeding line of Oryza sativa (2n=24, AA) and a wild species, O. minuta (2n=48, BBCC). F1 hybrids performance were intermediate between the parents. The F1 hybrids had 36 chromosomes indicating having 12 chromosome A from O. sativa and 12 B and 12 C from O. minuta. THE BACK CROSS progenies had different chromosome number indicating abnormal meiosis of the hybrids and back cross progenies. Plant with 2n=24 and 25 chromosomes were obtained in BC4FI. The hybrids and backcross progenies were susceptible to bacterial leaf blight (BB). However, several of the 2n=24 plants derived resistant plant to bacterial leaf blight race 1 of the Philippines races. The gene is different from introgressed gene intio rice from O. longistaminata ( Xa21) and from O. Minuta Acc. 101141   I Key words: Oryza minuta, MAALS, Bacterial leaf blight
Progress of Rice Improvement through Recurrent Selection Abdullah, Buang
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 37, No 3 (2009): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Improved rice varieties play an important role in increasing rice production, through raising and/or stabilizing rice productivity.  Thus, it is urgent to develop improved rice varieties.  Selection method is an important step in rice improvement.  Implementation of recurrent selection in rice improvement was aimed to increase the efficiency and insurance in the success of improvement.  Since 2004, Indonesian Institute for Rice Research has applied recurrent selection in breeding program. Promising lines of earliness and high yield potential were developed in relatively short time.  A number of advanced lines has been produced and will be further evaluated, and a number of populations has been selected and used as base populations for recurrent selection in rice improvement program.   Key words:  breeding, earliness, high yield rice, improvement
Hubungan Karakter Daun dengan Hasil Padi Varietas Unggul Wahyuti, Titin Budi; Purwoko, Bambang Sapta; Junaedi, Ahmad; ., Sugiyanta; Abdullah, Buang
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 41, No 3 (2013): JURNAL AGRONOMI INDONESIA
Publisher : Bogor Agricultural University

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Abstract

Penelitian ditujukan untuk mempelajari  hubungan karakter daun dengan hasil padi varietas unggul, dan dilaksanakandi kebun percobaan Balai Besar Penelitian Tanaman Padi, Muara, Bogor pada bulan Desember 2010 sampai Juni 2011.Percobaan menggunakan rancangan kelompok lengkap teracak dengan empat ulangan dan menggunakan 12 padi varietasunggul sebagai perlakuan. Varietas yang digunakan adalah Rojolele dan Pandan Wangi (varietas unggul lokal/VUL); IR64 dan Ciherang (varietas unggul baru/VUB); Fatmawati, Cimelati, galur BP360 dan B11143 (padi tipe baru/PTB);serta Maro, Rokan, SL-8 SHS, dan PP1 (hibrida). Hasil penelitian menunjukkan Galur B11143, Maro, Cimelati, dan Rokanmemberikan hasil gabah tertinggi. Tingginya hasil berhubungan dengan karakter sudut tiga daun bagian atas, luas daunbendera, kandungan klorofil, dan kandungan gula daun bendera. Karakter sudut tiga daun bagian atas dan luas daunbendera berkorelasi negatif dengan hasil. Kandungan klorofil dan gula daun bendera tahap berbunga dan pengisian bijiberkorelasi positif dengan hasil.Kata kunci: hasil, karakter daun, padi varietas unggul
PERFORMANCE OF GRAIN QUALITY AND AROMA OF AROMATIC NEW PLANT TYPE PROMISING RICE LINES Lestari, Angelita Puji; Abdullah, Buang; Junaedi, Ahmad; Aswidinnoor, Hajrial
Indonesian Journal of Agricultural Science Vol 12, No 2 (2011): October 2011
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Grain quality and aroma are important characteristics of rice that affect consumer acceptance. This research was conducted to study the grain quality and aroma of aromatic new plant type (NPT) promising rice lines. Thirty five lines as well as Ciherang and Sintanur varieties were planted at Bogor and Pusakanagara, West Java in the dry season (DS) 2009 and wet season (WS) 2009. Three methods, i.e. leaf aroma tested with KOH, rice aroma tested in the test tube, and cooked rice aroma test, were used to evaluate the aroma of the lines. The results showed that line B11742-RS*2-3-MR-34-1-2-1 was aromatic identified using different methods. The line had long, slender, and small chalkiness grains, high percentage of head rice, high amylose, and hard texture. Lines IPB 140-F-6, B11249-9C-PN-3-3-2-2-MR-1, and B11955-MR-84-1-4 also had a high aroma score and grain yield. Testing leaf aroma with KOH can be used as early selection method in breeding program for aromatic lines. Lines derived from aromatic parents from highlands of South Sulawesi did not show consistent aroma under three testing methods. Those tested lines had good grain quality, both physical and cooked rice quality.
Asosiasi antara marka SSR dengan ketahanan terhadap wereng batang coklat pada varietas dan calon galur harapan padi Chaerani, Chaerani; Utami, Dwinita W; Hidayatun, Nurul; Abdullah, Buang; Suprihatno, Bambang
Jurnal Entomologi Indonesia Vol 11, No 1 (2014)
Publisher : Perhimpunan Entomologi Indonesia

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Abstract

Development of resistant rice varieties to brown planthopper (BPH, Nilaparvata lugens Stål) must be countinuously conducted to overcome the spatial and temporal genetic change in BPH into more virulent ones which can adapt to resistance rice. The availability of linked molecular markers with BPH resistant trait can speed up selection of hybridization progenies. Close-linked markers with trait of interest can be identified using association studies between markers and phenotypic data of unrelated individuals without the need to generate hybridization progenies as an alternative method to linkage studies which use mapping population. The objective of this study was to analyze the association of rice simple sequence repeat (SSR) markers with resistance to BPH. Forty-four rice varieties and promising lines with known resistance degree to BPH biotype 3 were assessed using 30 rice microsatellite markers previously mapped in the rice chromosomal regions with effects on resistance to BPH. Association test between SSR markers and BPH resistance scores revealed eight markers (RM17, RM38, RM125, RM144, RM250, RM287, RM328, dan RM536) which were significantly associated with BPH resistance (R2=0,18 to 0,89; P&lt;0,05). Twenty-one varieties and 11 promising lines were detected to contain SSR alleles that were associated with BPH resistance. Marker RM17 was detected in 15 varieties and 11 promising lines which were scored as resistant and moderately resistant to BPH and therefore it is potentially the most useful as early diagnostic marker to detect BPH resistance alleles. Nevertheless, to obtain SSRs that can be used as reliable selection markers for BPH resistance, marker analysis in segregating populations for the eight markers is still needed as well as associtaion test of more SSR markers widely distributed in rice chromosomes.
STUDY OF MILLING PROCESS AND ITS EFFECT ON VITAMIN B1 AND FOLIC ACID CONTENTS ON LOWLAND RICE PROMISING LINES Indrasari, Siti Dewi; Ardhiyanti, Shinta Dewi; Abdullah, Buang
Indonesian Journal of Agricultural Science Vol 15, No 2 (2014): October 2014
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Rice is an important source of vitamin B1 and folic acid, but cultivated rice cultivars contain low of these nutrient. Breeding program had been conducted and several advanced lines with a high vitamin B and folic acid contents were found. This research aimed to study the contents of vitamin B1 and folic acid in the brown rice and milled rice of fifteen promising lowland rice lines. Dried paddy (14% moisture content) were husked in the mini husker (Satake THU 35A) to obtain brown rice and milled rice by using abrasive-type mini polisher (Satake TM-05, 1,450 RPM for 2 minutes). Vitamin B1 and folic acid in the brown rice and milled rice were determined by using HPLC equipped with fluorescence detector for vitamin B1 and UV detector for folic acid. Four out of fifteen rice lines studied showed a unique characteristic of vitamin B1 content in the brown rice and milled rice. Rice lines which have highest vitamin B1 in the brown rice were B10267-4-PN-6-2-3-2-2-2-3-3-2 (3.03 mg 100 g-1), B10876H-MR-2 (2.13 mg 100 g-1), B10531E-KN-1-2-PN-1-4-2 (1.33 mg 100 g-1) and B12411E-RS*-1-2-1 (1.21 mg 100 g-1). However after milling, 43-92% of their vitamin B1 were lost; the least loss was observed in B12411E-RS*-1-2-1 (1.21 mg 100 g-1) which was only  4%. Three out of fifteen rice lines studied has highest folic acid in brown rice, i.e. BP400G-PN-12-3-6 (158.5 µg 100 g-1), B10876H-MR-2 (152.0 µg 100 g-1), and B11742-RS*-2-3-MR-5-5-1-Si-1-3 (100.0 µg 100 g-1). After milling the line B10876H-MR-2 had the highest folic acid content (52.0 µg 100 g-1), much higher than that of Ciherang variety as a control. This study indicates the importance of having a suitable milling instrument to minimize loss of vitamin B1 and folic acid. The study also suggests the importance of creating new rice cultivars that have high nutrient and resistant to milling process.
Asosiasi antara marka SSR dengan ketahanan terhadap wereng batang coklat pada varietas dan calon galur harapan padi Chaerani, Chaerani; Utami, Dwinita W.; Hidayatun, Nurul; Abdullah, Buang; Suprihatno, Bambang
Jurnal Entomologi Indonesia Vol 11, No 1 (2014): April
Publisher : Perhimpunan Entomologi Indonesia

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Abstract

Development of resistant rice varieties to brown planthopper (BPH, Nilaparvata lugens Stål) must be countinuously conducted to overcome the spatial and temporal genetic change in BPH into more virulent ones which can adapt to resistance rice. The availability of linked molecular markers with BPH resistant trait can speed up selection of hybridization progenies. Close-linked markers with trait of interest can be identified using association studies between markers and phenotypic data of unrelated individuals without the need to generate hybridization progenies as an alternative method to linkage studies which use mapping population. The objective of this study was to analyze the association of rice simple sequence repeat (SSR) markers with resistance to BPH. Forty-four rice varieties and promising lines with known resistance degree to BPH biotype 3 were assessed using 30 rice microsatellite markers previously mapped in the rice chromosomal regions with effects on resistance to BPH. Association test between SSR markers and BPH resistance scores revealed eight markers (RM17, RM38, RM125, RM144, RM250, RM287, RM328, dan RM536) which were significantly associated with BPH resistance (R2=0,18 to 0,89; P&lt;0,05). Twenty-one varieties and 11 promising lines were detected to contain SSR alleles that were associated with BPH resistance. Marker RM17 was detected in 15 varieties and 11 promising lines which were scored as resistant and moderately resistant to BPH and therefore it is potentially the most useful as early diagnostic marker to detect BPH resistance alleles. Nevertheless, to obtain SSRs that can be used as reliable selection markers for BPH resistance, marker analysis in segregating populations for the eight markers is still needed as well as association test of more SSR markers widely distributed in rice chromosomes.