Asadatun Abdullah
Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Bogor

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Aktivitas Antioksidan dan Komponen Bioaktif Kerang Pisau (Solen spp) Nurjanah, Nurjanah; Izzati, Laili; Abdullah, Asadatun
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 3 (2011): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Kerang pisau (Solen spp) merupakan salah satu jenis moluska dari kelas Bivalva yang banyak ditemukan di daerah pantai berlumpur di perairan Kabupaten Pamekasan Madura.Tujuan penelitian adalah menentukan aktivitas antioksidan dan komponen bioaktif yang terkandung dalam kerang pisau. Pengujian yang dilakukan meliputi analisis aktivitas antioksidan dengan metode DPPH, dan uji fitokimia. Kerang pisau memiliki aktivitas antioksidan yang terlihat dari nilai LC50 yang diperoleh. Nilai LC50 dari ekstrak kloroform sebesar 2008,52 ppm, ekstrak etil asetat 1593,87 ppm dan ekstrak metanol 1391,08 ppm. Ekstrak kasar kerang pisau mengandung alkaloid, steroid, dan flavonoid. Kerang pisau dapat dinyatakan sebagai salah satu jenis kerang-kerangan penghasil senyawa antioksidan dan dapat dikembangkan, baik dalam bidang pangan maupun farmasi.  Kata kunci:  Aktivitas antioksidan, kerang pisau (Solen spp), senyawa bioaktif. Razor clams (Solen spp) were commonly found in muddy Pamekasan waters, Madura. This research was conducted to determine the potential of razor clams as one type of shellfish producing antioxidant compounds. The tests included quantitative test of antioxidant activity by DPPH method, and phytochemical test. Razor clams has antioxidant activity as showed on the LC50 values obtained. LC50 value of chloroform, ethyl acetate, and methanol extract were 2008 2 ppm, 1593,87 ppm and 1391,08 ppm, respectively. The crude extract of razor clams is contained 3 bioactive components in the forms of alkaloids, steroids,and flavonoids. Razor clams can be expressed as one type of shellfish-producing antioxidant compounds and can be developed, both in food and pharmaceutical fields.  Key words: antioxidant activity, razor clams (Solen spp), bioactive compound.
AKTIVITAS ANTIOKSIDAN DAN KOMPONEN BIOAKTIF KEONG IPONG-IPONG (Fasciolaria salmo) Nurjanah, .; Abdullah, Asadatun; Apriandi, Azwin
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 14, No 1 (2011): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Departement of Aquatic Product Technology

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Abstract

Ipong-ipong snail (Fasciolaria salmo) is one of sea water gastropods that have not been utilized optimally. People believe that the snail has the ef cacies for increasing stamina and vitality. However, scienti c data supporting the ef cacy of this snail has not been elucidated yet. The purposes of this research was determined the yield, chemical content (water, protein, fat, ash, acid insoluble ash and carbohydrates), bioactive components and antioxidant activity. The yield of shells, meat, and viscera were 69.69%, 22.08% and 8.22%, respectively. Watercontent, protein, fat, ash, acid insoluble ash and carbohydrates were 73.075%, 18.28%, 0.575%, 2.77%, 0.15% and 5.2%, respecitevely. Six bioactive components were detected i.e alkaloids, steroids, reducing sugars, carbohydrates, peptides and amino acids. Antioxidant activity using DPPH method obtained the result IC50 of the meat extract with chloroform, ethyl acetate and methanol solvent were 9210 ppm, 6825 ppm, 1513.8 ppm, respectively. Meanwhile for viscera extract were. 2825 ppm, 4600 ppm and 994,47 ppm, respectively.Keywords: antioxidants, bioactives, Fasciolaria salmo, proximate, yield.
AUTENTIKASI TUNA STEAK KOMERSIAL DENGAN METODE PCR-SEQUENCING Abdullah, Asadatun; Nurjanah, .; Kurnia, Nanang
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 14, No 1 (2011): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Departement of Aquatic Product Technology

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Abstract

Tuna is one of the  shery commodities which are susceptible to mislabeling and substituted with similarspecies, but lower price. Consumer as a purchaser will incur a loss (economical fraud) so it is needed a way toovercome these problems. This study aimed to optimized extraction of DNA obtained from the tuna and tunaexporter companies of modern markets, identi cation of DNA-based species-speci c primers with a target genecyt b, and characterization of DNA tuna authentication results. This study consisted of several steps beginning with the characterization of tuna, DNA extraction using CTAB method and Vivantis kit, ampli cation by PCR, electrophoresis, and nucleotide sequencing. The samples tested were successfully extracted and ampli ed with the appropriate size of 750 base pairs. PCR sequencing using cyt b gene targets resulted in the identi cation of tuna raw material. PCR sequencing of the nucleotide sequence of results which have been  tted to the NCBI data, which does not show any fraud in the form of substitution with other species. Species of yellow  n (Thunnus albacore), Albacore (Thunnus alalunga), big eye (Thunnus obesus) and blue  n (Thunnus macoyyi) has the highest homology i.e 99%, 99%, 99%, 100%, respectively.Keywords: authentication, cyt b, mt-DNA, PCR, tuna’s steaks
Physical and Chemical Characteristics of Agar Bacto with Addition Of Chitosan Abdullah, Asadatun; Suptijah, Pipih; Suwandi, Ruddy
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 11, No 1 (2008): Buletin Teknologi Hasil Perikanan
Publisher : Departement of Aquatic Product Technology

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Abstract

Agar adalah bentuk koloid dari suatu polisakarida kompleks yang diekstrak dari beberapa kelompok alga merah (Rhodophyceae). Salah satu pemanfaatan dari agar adalah sebagai media untuk pertumbuhan mikroba. Agar bakto adalah salah satu medium kultur yang digunakan untuk membantu menggelifikasi berbagai jenis larutan medium atau larutan kaldu (broth). Penambahan kitosan sebagai absorben pada proses pemurnian agar diharapkan dapat menghasilkan agar yang murni (refine agar) yang dapat digunakan untuk agar bakto sebagai media pertumbuhan mikroba. Tujuan dari penelitian ini adalah memodifikasi berbagai konsentrasi kitosan dan waktu proses absorbsi dalam pembuatan agar bakto sebagai media pertumbuhan mikroba. Penelitian dibagi menjadi dua tahap, yang pertama adalah tahapan proses isolasi kitin dan deasetilasi kitin menjadi kitosan dan penelitian utama yang dilakukan yaitu pembuatan agar bakto dengan menambahkan berbagai konsentrasi kitosan (0,5%, 1%, 1,5%) sebagai absorben terhadap agar yang dihasilkan dari Gracilaria sp. dan agar batang. Perlakuan kedua yang diberikan yaitu waktu proses absorbsi setelah dilakukan penambahan kitosan. Proses absorbsi yang dipilih 0 menit, 15 menit, 30 menit, dan 45 menit. Kombinasi perlakuan paling optimum untuk agar bakto berbahan baku Gracilaria sp. adalah pada konsentrasi kitosan 1% dengan lama pemanasan lanjutan ekstraksi selama 45 menit. Kombinasi perlakuan paling optimum untuk agar bakto berbahan baku agar batang adalah pada konsentrasi kitosan 0,5% dengan tanpa pemanasan lanjutan.Kata kunci: agar bakto, Gracilaria sp. , kitosan
Karakteristik Fisik dan Kimia Tepung Cangkang Kijing Lokal (Pilsbryoconcha exilis) Abdullah, Asadatun; nurjanah, Nurjanah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 13, No 1 (2010): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Departement of Aquatic Product Technology

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Abstract

Local mussel (Pilsbryoconcha exilis) is one aquatic commodity that has high enough potential. Mussel shell is solid waste that has not been used optimally. The purpose of this research is to study the physical and chemical characteristics shells and mussel shell flour with the different of size and body length. The observed parameters include the physical characteristics of shells, yield, chitin, flour yield, degree of white, proximate contents, pH, minerals and mineral solubility determination of mussel shell flour. Mussel obtained from waters Situ Gede has length between 72-103 mm, 31-47 mm high and 13-34 mm thick. Mussel shells in all size contained chitin ranging from 0,72% to 0,75%. Mussel shell flour measuring < 90 mm 20% larger than the size of mussel ≥ 90 mm. Mussel shell flour measuring < 90 mm has a value of 5% degrees whiter than white shell size ≥ 90 mm. Shell flour has a water content between 1,19-1,2%, 93,14-93,34% of ash, 1,85-2,31% of protein, 0,66-0,72% of fat, carbohydrate by difference 2,62-2,94% with a pH range of 8,5-8,9. Mussel shell flour has a mineral content, respectively from the largest which are calcium, phosphorus and magnesium. The calcium and phosphorus mussels flour shell has an optimal value for solubility at pH 2. Mussel shells contained chitin ranged from 0,72% to 0,75%. Mussel shell size difference gave a significantly different effect on the physical characteristics of the mussel shell flour produced but did not influence significantly different to the chemical characteristics of the mussel shell flour produced.
Inhibitors Screening of β-lactamase Axinella Sponge Simbion Bacteria sp. Abdullah, Asadatun; Hardjito, Linawati; Ernawati, .; Abdillah, Fatimah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 11, No 2 (2008): Buletin Teknologi Hasil Perikanan
Publisher : Departement of Aquatic Product Technology

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Abstract

Salah satu jenis antibiotik yang umum digunakan dalam pengobatan adalah β-laktam. Saat ini terdapat banyak bakteri patogen pada manusia yang resisten terhadap antibiotik β-laktam. Bakteri patogen yang resisten terhadap antibiotik β-laktam adalah bakteri yang mampu memproduksi enzim β-laktamase. Tujuan penelitian ini adalah menapis produk alami inhibitor β-laktamase dari mikroorganisme simbion Axinella sp. Berdasarkan penelitian yang dilakukan sebelumnya, telah diisolasi dan diidentifikasi secara biokimia 7 isolat bakteri simbion sponge Axinella sp. Penapisan inhibitor β-laktamase dilakukan dengan metode agar difusi menggunakan bakteri target E.coli dan S.aureus resisten ampisilin koleksi Laboratorium Bioteknologi Hasil Perairan. Pengukuran resistensi terhadap E.coli dan S.aureus dilakukan pada konsentrasi ampisilin >32 μg/ml. Dari 7 isolat bakteri simbion yang ditapis secara intraseluler dan ekstraseluler, 4 isolat pada ekstraselulernya positif menghambat pertumbuhan bakteri patogen E.coli dan S.aureus. 4 bakteri simbion tersebut adalah Bacillus sp., Halomonas sp., Alteromonas sp. dan Alteromonas sp. Identifikasi molekuler bakteri simbion dan karakterisasi golongan senyawa inbitor β-laktamase sedang dalam proses penelitian.Keywords : bakteri simbion Axinella sp., inhibitor β-laktamase, E.coli, S.aureus
AKTIVITAS ANTIOKSIDAN DAN KOMPONEN BIOAKTIF KEONG IPONG-IPONG (Fasciolaria salmo) Nurjanah, .; Abdullah, Asadatun; Apriandi, Azwin
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 14, No 1 (2011): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.656 KB) | DOI: 10.17844/jphpi.v14i1.3425

Abstract

Ipong-ipong snail (Fasciolaria salmo) is one of sea water gastropods that have not been utilized optimally. People believe that the snail has the ef? cacies for increasing stamina and vitality. However, scienti? c data supporting the ef? cacy of this snail has not been elucidated yet. The purposes of this research was determined the yield, chemical content (water, protein, fat, ash, acid insoluble ash and carbohydrates), bioactive components and antioxidant activity. The yield of shells, meat, and viscera were 69.69%, 22.08% and 8.22%, respectively. Watercontent, protein, fat, ash, acid insoluble ash and carbohydrates were 73.075%, 18.28%, 0.575%, 2.77%, 0.15% and 5.2%, respecitevely. Six bioactive components were detected i.e alkaloids, steroids, reducing sugars, carbohydrates, peptides and amino acids. Antioxidant activity using DPPH method obtained the result IC50 of the meat extract with chloroform, ethyl acetate and methanol solvent were 9210 ppm, 6825 ppm, 1513.8 ppm, respectively. Meanwhile for viscera extract were. 2825 ppm, 4600 ppm and 994,47 ppm, respectively.Keywords: antioxidants, bioactives, Fasciolaria salmo, proximate, yield.
ANALISIS KANDUNGAN LOGAM BERAT DAGING KIJING LOKAL (Pilsbryoconcha exilis) DARI PERAIRAN SITU GEDE, BOGOR Nurjanah, Nurjanah; Sembiring, Rodieser; Abdullah, Asadatun
Asian Journal of Innovation and Entrepreneurship Vol 1, No 01 (2012): January 2012
Publisher : http://journal.uii.ac.id/

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Abstract

The purpose of this research was to examine the content of heavy metals Hg, Cd, and Pb of small and large clams over a period of two months (May and July). In this study, depuration system was applied in an effort to reduce the content of heavy metals. The study was done with inventorisation of the sample area, analysis of the characteristics of clams, operation of depuration system, and analysis of content of Hg, Cd, and Pb. Clam in Situ Gede waters showed heavy metal content of mercury and cadmium that were not detected in meat during the period of two months (May and July) both in large and small clams. Content of lead in May was higher than in July. The average lead in waters Situ Gede during the two periods amounted to 1.34 ppm in small clams and 1.44 ppm in large clams. Depuration system could reduce Pb contents in small clams 0.0861 ppm after 10 days and 0.1506 ppm after 20 days and in large clam 0.0513 ppm after 10 days and 0,0835 ppm after 20 days.
AKTIVITAS ANTIOKSIDAN DAN KOMPONEN BIOAKTIF SEMANGGI AIR (MARSILEA CRENATA) Nurjanah, Nurjanah; Azka, Aulia; Abdullah, Asadatun
Asian Journal of Innovation and Entrepreneurship Vol 1, No 03 (2012): September 2012
Publisher : UII

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/ajie.vol1.iss3.art2

Abstract

Water clover (Marsilea crenata) is one type of aquatic plants. Plants that fall into these ferns are found in the rice field, ponds, lakes, swamps and rivers. This plant has a very distinctive morphology of its leaves resemble an umbrella shape that is composed of four petals facing child leaves. In the area of young clover Java is widely used as food. Utilization of clover water not only as food, water clover leaves and stems can also be used as drug . A research about peculiar properties of water clover for human health is very important to do, like antioxidant activity assay and qualitative bioactive compounds assay. The objectives of this research were to determine content, chemical compounds (water, lipid, protein, ash and crude fiber), antioxidant activity and bioactive compounds of water clover. Methods that used in this research were proximate analysis, quantitative antioxidant activity assay using DPPH method, and phytochemical method. Water clover planted first in Laboratorim Characteristics of Raw Water Results, Aquatic Product Technology, FPIK, IPB. Crude extracts from water clover have antioxidant activity that measured as IC50 value. The IC50 value from chloroform extract, ethyl acetate extract, and methanol extract of water clover were 1285,39 ppm; 915,03 ppm dan 634,73 ppm, respectively. The crude extracts of water clover contained 6 bioactive compounds, i.e alkaloid, steroid/triterpenoid, flavonoid, carbohydrate, reducing sugar and free amino acid. These compounds suspected have positive physiology functions for human health, so that water clover can be used for raw material of nutraceutical. Keywords: antioxidant activity, bioactive compound, water clover (Marsilea crenata)
Karakteristik Fisik dan Kimia Tepung Cangkang Kijing Lokal (Pilsbryoconcha exilis) Abdullah, Asadatun; nurjanah, Nurjanah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 13, No 1 (2010): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (75.836 KB) | DOI: 10.17844/jphpi.v13i1.1215

Abstract

Local mussel (Pilsbryoconcha exilis) is one aquatic commodity that has high enough potential. Mussel shell is solid waste that has not been used optimally. The purpose of this research is to study the physical and chemical characteristics shells and mussel shell flour with the different of size and body length. The observed parameters include the physical characteristics of shells, yield, chitin, flour yield, degree of white, proximate contents, pH, minerals and mineral solubility determination of mussel shell flour. Mussel obtained from waters Situ Gede has length between 72-103 mm, 31-47 mm high and 13-34 mm thick. Mussel shells in all size contained chitin ranging from 0,72% to 0,75%. Mussel shell flour measuring < 90 mm 20% larger than the size of mussel ? 90 mm. Mussel shell flour measuring < 90 mm has a value of 5% degrees whiter than white shell size ? 90 mm. Shell flour has a water content between 1,19-1,2%, 93,14-93,34% of ash, 1,85-2,31% of protein, 0,66-0,72% of fat, carbohydrate by difference 2,62-2,94% with a pH range of 8,5-8,9. Mussel shell flour has a mineral content, respectively from the largest which are calcium, phosphorus and magnesium. The calcium and phosphorus mussels flour shell has an optimal value for solubility at pH 2. Mussel shells contained chitin ranged from 0,72% to 0,75%. Mussel shell size difference gave a significantly different effect on the physical characteristics of the mussel shell flour produced but did not influence significantly different to the chemical characteristics of the mussel shell flour produced.