Articles

Preparasi antigen KHV untuk pencegahan infeksi KHV pada ikan koi (Cyprinus carpio) ., E. Sulistiyowati; ., St.S. Yasin; ., W. Suharni; ., S.R. Setyaningsih; ., U. S Kuba; ., Saribanong; ., Hasmi; ., St. Narwiyani; ., Suriati; ., Widodo
Hemera Zoa Vol 1, No 2 (2010): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

Infeksi Koi Herpes Virus merupakan penyakit sangat serius yang menyerang ikan mas dan koi. Penyakit ini menyebabkan kematian massal sebesar 80-95% dari total populasi dan menimbulkan kerugian ekonomi yang sangat besar. Tujuan dari studi ini adalah untuk mendapatkan antigen HlV yang dapat digunakan untuk mencegah infeksi KHV. Metode preparasi antigen dilalakukan dengan membuat suspensi organ 10 % dari seluruh organ target ikan yang positif terinfeksi KHV, kemudian dipekatkan sehingga menghasilkan konsentrat organ (antigen). Sebagian dari konsentrat organ (antigen) yang dihasilkan, di-inaktif-kan dengan formaldehyde 0,3%, Kemudian disuntikkan pada ikan koi sehat (vaksinasi). Penyuntikan dilakukan sebanyak 3 kali dalam kurun waktu 4 minggu. Satu minggu setelah penyuntikan ke-3 dilakukan uji tantang. uji tantang dilakukan dengan menyuntikan virus aktif KHV dengan dosis 0,1 ml secara intraperitoneal pada koi yang telah divaksin. Sebagai kontrol, dilakukan juga penyuntikan pada koi yang tidak diberi perlakuan. Hasil uji tantang menunjukkan bahwa semua ikan yang divaksinasi dengan ketiga metode preparasi antigen tidak menunjukkan gejala klinis terinvaksi KHV.
PENGARUH TEGANGAN ELEKTROPORASI TERHADAP EFISIENSI TRANSFORMASI PLAMID pND968 BAKTERI ASAM LAKTAT KE E. coli HB101 1) [The Effect of Electroporation Voltage on Transformation Efficiency of Plasmid pND968 of Lactic Acid Bacteria Into E. coli HB101] ., Widodo
Jurnal Teknologi Dan Industri Pangan Vol 13, No 2 (2002): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/4349

Abstract

PENGARUH TEGANGAN ELEKTROPORASI TERHADAP EFISIENSI TRANSFORMASI PLAMID pND968 BAKTERI ASAM LAKTAT KE E. coli HB101 1)   [The Effect of Electroporation Voltage on Transformation Efficiency of  Plasmid pND968  of Lactic Acid Bacteria Into E. coli HB101] Widodo 2) 1) Makalah dipresentasikan pada Seminar Nasional PATPI, Semarang 9-10 Oktober 2001 2) Laboratorium Teknologi Pengolahan Susu, Fakultas Peternakan UGM – Yogyakarta ABSTRACT   The research was conducted to examine the effect of different electroporation voltage on transformation efficiency of plasmid pND968. This was carried out by exposing the mixture of plasmid pND968 and competent cells of E. coli  HB101 on  a difference level of electroporation voltage at 1500, 2000 and 2500 Volts. After electroporation, the cells were resuspended in a recovery medium and then poured into ampicilin-containing LB agar. Transformants appeared after 48-72 hours were then recorded. The results showed that electroporation voltage of 2500 Volts with resistance 200 Ohm and capacitor 25 uF resulted in the highest electroporation efficiency at 7.72 x 104 CFU / ug DNA. Transformants confirmation by means of plasmid pND968 isolation showed that all of  the ampicillin-resistant transformants harbored the intended pND968 with the expected size. Key words : Electroporation, transformation efficiencies, and plasmid  
UPAYA PENINGKATAN KOMITMEN ORGANISASI ., Widodo
Jurnal Bisnis dan Ekonomi Vol 15, No 2 (2008): Vol. 15 No. 2 September 2008
Publisher : Jurnal Bisnis dan Ekonomi

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This study aim to strive improvement of commitment organization, with ability variables asesteems, trust, benefit the relation and quality of communications. The thing is expected at theheight of commitment of organization can trigger improvement of organization performance.Sample applied at this study is customer of supermarket, Then analytical technique appliesSPSS. This study expected can give contribution at expansion of science marketing and asconsideration bottom of decision making to increase commitment of organization.Key words: commitment, ability, esteems, trust, benefit the relation and quality ofcommunications
MODEL PENGEMBANGAN KEPUASAN KERJA DENGAN KEPUASAN KELUARGA ., Widodo
Jurnal Bisnis dan Ekonomi Vol 17, No 1 (2010): Vol. 17 No. 1 Maret 2010
Publisher : Jurnal Bisnis dan Ekonomi

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This study tested job satisfaction has a reciprocal relationship with family satisfaction. Itshows that work and family life are two things that can not be separated within anorganization.The population in this study is one of the units government institution in the city ofSemarang, as many as 123 people. Therefore, using the census because its population is limited.The analysis using t test and bivariate with software SPSS. The results of this study are expectedto develop human resource management and decision-making inputs, especially to increase joband family satisfaction.Keywords: job satisfaction, family satisfaction, job stressor, work involvement
UPAYA PENINGKATAN KINERJA SUMBER DAYA MANUSIA MELALUI KOMITMEN DAN OREINTASI BELAJAR ., Widodo
MEDIA EKONOMI Vol 9, No 1 (2009)
Publisher : MEDIA EKONOMI

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This study based on reserach gap commitment and learning orietation tohuman resources performance Contribution in this study is intervening variable smart workingThe respondents for this research were the 95 personnel hospital inSemarang. The data were then analyzed using Multiple regression SPSSoptware Windows SPSSFinding result of this study indicates that in the effort increasing humanresources performance of main preference is trought smart working builtlearning orientationKey word : Smart working, Learning orientation, commitment, Human resourcesperformance
Preparasi antigen KHV untuk pencegahan infeksi KHV pada ikan koi (Cyprinus carpio) ., E. Sulistiyowati; ., St.S. Yasin; ., W. Suharni; ., S.R. Setyaningsih; ., U. S Kuba; ., Saribanong; ., Hasmi; ., St. Narwiyani; ., Suriati; ., Widodo
Hemera Zoa Vol 1, No 2 (2010): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

Infeksi Koi Herpes Virus merupakan penyakit sangat serius yang menyerang ikan mas dan koi. Penyakit ini menyebabkan kematian massal sebesar 80-95% dari total populasi dan menimbulkan kerugian ekonomi yang sangat besar. Tujuan dari studi ini adalah untuk mendapatkan antigen HlV yang dapat digunakan untuk mencegah infeksi KHV. Metode preparasi antigen dilalakukan dengan membuat suspensi organ 10 % dari seluruh organ target ikan yang positif terinfeksi KHV, kemudian dipekatkan sehingga menghasilkan konsentrat organ (antigen). Sebagian dari konsentrat organ (antigen) yang dihasilkan, di-inaktif-kan dengan formaldehyde 0,3%, Kemudian disuntikkan pada ikan koi sehat (vaksinasi). Penyuntikan dilakukan sebanyak 3 kali dalam kurun waktu 4 minggu. Satu minggu setelah penyuntikan ke-3 dilakukan uji tantang. uji tantang dilakukan dengan menyuntikan virus aktif KHV dengan dosis 0,1 ml secara intraperitoneal pada koi yang telah divaksin. Sebagai kontrol, dilakukan juga penyuntikan pada koi yang tidak diberi perlakuan. Hasil uji tantang menunjukkan bahwa semua ikan yang divaksinasi dengan ketiga metode preparasi antigen tidak menunjukkan gejala klinis terinvaksi KHV.
Efektifitas Aplikasi In-vitro Rizobakteri Sebagai Agen Antagonis Layu Fusarium pada Pisang Rajabulu/AAB di Rumah Kaca ., Kasutjianingati; Poerwanto, Roedhy; ., Widodo; Khumaida, Nurul; Efendi, Darda
Jurnal Hortikultura Indonesia Vol 2, No 1 (2011): Jurnal Hortikultura Indonesia
Publisher : Perhimpunan Hortikultura Indonesia

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Research was conducted on a banana cultivar of Rajabulu/AAB inside the green house. This research was aimed to study the effect of rhizobacteria and the ability of in vitro bacterization to prevent FOC. This study was conducted using split plot design. The main plot was rhizobacteria treatments (P. fluorescens ES-32, B. subtilis SB-3, a mix of both types and without bacteria), sub plots consist of in vitro (2 weeks and 1 week prior to application) and in vivo (during acclimatization) bacterization. All treatments were repeated 3 times, each consist of 7 polibags. The result of rhizobacteria treatment (B. subtilis SB3 or P. fluorecens ES32) was able to reduce the Disease Severity (Rhizome Discoloration) caused by Fusarium oxysporum f.sp cubense (FOC). The incorporation of mixed rhizobacteria provided significant effect suppressing the infestation of FOC. The application of mix bacteria was better than single treatment.Keywords: B. subtilis, P. fluorecens, rhizome discoloration, fusarial wilt, suppressing
Keragaman Fusarium pada Rizosfer Tanaman Kacang Panjang dan Peranannya bagi Pertumbuhan Tanaman Sutrisni, Rahmi; ., Widodo
Jurnal Fitopatologi Indonesia Vol 8, No 5 (2012)
Publisher : Jurnal Fitopatologi Indonesia

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Fusarium wilt caused by Fusarium oxysporum f.sp. tracheiphilum is one of the important diseases on yard long bean. Besides of its capability of causing diseases in plants, some species of Fusarium are non pathogenic and able to control Fusarium wilt as naturally occured in disease suppressive soils. The objective of this study was to observe the rhizosphere Fusarium diversity and its role on the plant growth of yard long bean. Out of 56 isolates collected during the study, four species were identified, including F. oxysporum (39.3%), F. semitectum (28.6%), F. longipes (12.5%), F. solani (5.3%), and one unidentified Fusarium sp. (14.3%). The highest diversity index was obtained from the field planted with corn as previous crop. In general, 56 isolates tested could be differentiated into 3 groups based on their effects on plan growth, i.e.: deleterious that caused wilting or growth suppressive without wilting (50.0%), neutral that no significantly difference growth as untreated (41.1%), and growth promoting (8.9%). All three isolated Fusarium causing plant wilting identified as F. oxysporum, while isolates with growth promoting effect were identified as F. oxysporum (4 isolates) and F. solani (1 isolate). These five selected growth promoting Fusarium also suppressed the wilt disease and increased the plant growth when co inoculated with wilt pathogen of yard long bean, F. oxysporum f.sp. tracheiphillum.Key words: diversity, Fusarium, long yard bean, rhizosphere
Laporan Pertama tentang Penyakit Busuk Arang pada Kacang Tanah Wiyono, Suryo; ., Widodo
Jurnal Fitopatologi Indonesia Vol 8, No 2 (2012)
Publisher : Jurnal Fitopatologi Indonesia

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Peanut charcoal rot disease was first observed in Bogor,West Java in June 2010, and Serang, Banten in January 2012. Infected plants in the field showed root and stem rot, discoloration of stems and/or branches. Severe infection may cause wilting of foliage then plant dried out. Based on microscopic observation Macrophomina phaseolina was constantly associated with diseased plants from those two areas. Hot temperature and low rainfall might favour disease development. This is the first report of charcoal rot disease of peanut in West Java and Banten. Key words: charcoal rot, Macrophomina phaseolina, peanut
Penyakit Keriting Daun Pepaya yang Disebabkan oleh Cladosporium cladosporioides ., Widodo; Wiyono, Suryo
Jurnal Fitopatologi Indonesia Vol 8, No 1 (2012)
Publisher : Jurnal Fitopatologi Indonesia

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Leaf curling and chlorotic spot on young leaves of papaya type California was observed in Bogor, Kebumen, and Bekasi. Conidium of Cladosporium cladosporioides was found associated with diseased tissue based on microscopic examination. To our knowledge, this is the first report of C. cladosporioides causing papaya leaf curling in Indonesia. Key words: chlorotic, leaf curl disease, papaya disease, scab