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Kandidat Vaksin Potensial Streptococcus agalactiae untuk Pencegahan Penyakit Streptococcosis pada Ikan Nila (Oreochromis niloticus) (POTENTIAL VACCINE CANDIDATE OF STREPTOCOCCUS AGALACTIAE FOR PREVENT STREPCOCOCOSIS ON NILA TILAPIA (OREOCHROMIS NILOTICUS) Hardi, Esti Handayani; ., Sukenda; Harris, Enang; Lusiastuti, Angela Mariana
Jurnal Veteriner Vol 14, No 4 (2013)
Publisher : Jurnal Veteriner

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Abstract

The effectiveness of Streptococcus agalactiae vaccine in tilapia (Oreochromis niloticus) was evaluatedfor prevention of streptococcal disease. The vaccine was prepared using formalin-killed whole cell andconcentrated extracellular products/ECP (62.3 and 55.8 kDa) of â-haemolityc isolate and 62.3; 55,8 and51.8 kDa protein of  non-haemolityc ECP of S. agalactiae.  Vaccination and challenged (103 colony-formingunits (CFU)/fish of â-haemolityc and 105 CFU/fish of non-haemolityc S. agalactiae) trial was conducted byintraperitonial (IP) injection into fish with average body weight of 15 g.  Fish were vaccinated with wholecell, ECP and mixed (whole cell and ECP) vaccine.  Tilapia vaccinated with whole cell of â-haemolitycisolate had a relative percent survival (RPS) rates higher than those of ECP â-haemolityc vaccine. However,fish  vaccinated with mixed (whole cell and ECP) of â-haemolityc has a better protection rates as comparedto those of two type of S. agalactie infection. Whereas those vaccinated with mixed (whole cell non-haemolitycand ECP of â-haemolityc) vaccine has protection rate of 79% from â-haemolityc and 42% from non-haemolitycinfection.  Tilapia vaccinated with whole cell of non-haemolityc was only able to protect fish from non-haemolityc infection and was unable to protect fish from other types.  Tilapia vaccinated with ECP non-haemolityc had a worse RPS than others vaccines in which mix whole cell and ECP vaccine of non-haemolitychad a protection 50-56% from  S. agalactiae infection. Whereas vaccinated with mixed (whole cell â-haemolityc and ECP of non-haemolityc) vaccine showed a better to protect from â-haemolityc than non-haemolityc infection.  It showed thatvaccination with mixed (whole-cell and extracellular product)  vaccineof S. agalactiae â-haemolityc  was more effective to protect tilapia against Streptococcosis.
Prototipe Perangkat Lunak Studi dan Implementasi Sistem Antrian ., Sukenda; Laksono T., Roosaleh
Jurnal Informatika Vol 1, No 2 (2005)
Publisher : Universitas Kristen Maranatha

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Abstract

Queueing is one of the way to create discipline. In the queueinng, there are customers who demands service and owner of the service who provide those service. There is conflict of interest between those two because the customers do not want to queue too long and the service owner who wants to serve as many customers as possible. Hence, the service owner must be able to provide conditions such that customer queueing is not too long. To create good queue the observation of waiting time for customer, service time, and queueing time need to be closely examined. With those examination, the condition that satisfactory to cusomer and service owner with minimal extra cost. Based on those requirements, software called pro-que is engineered to create optimum condition for queueing. This system is built based on the object oriented method of software engineering known as OMT (Object Modelling Technique) by Rumbaugh.
Pemberian Fikosianin Spirulina Meningkatkan Jumlah Sel Darah, Aktivitas Fagositosis, dan Pertumbuhan Ikan Kerapu Bebek Juvenil (ADMINISTRATION OF SPIRULINA PHYCOCYANIN ENHANCES BLOOD CELLS, PHAGOCYTIC ACTIVITY AND GROWTH IN HUMPBACK GROUPER JUVENILE) Satyantini, Woro Hastuti; ., Sukenda; Harris, Enang; Utomo, Nur Bambang Priyo
Jurnal Veteriner Vol 15, No 1 (2014)
Publisher : Jurnal Veteriner

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The aim of this study was to investigate effects of Spirulina phycocyanin on the total  blood cell count,phagocytic activity, and growth of humpback grouper fish, Cromileptes altivelis juvenil.  Fishes were fedwith a diet containing   0, 150, 250, 350 dan 450 mg  phycocyanin per kg diet for four weeks and eachtreatment was triplicates.  Initial body weight  of  grouper was  8.46 ± 0.22 g with a density of 10 fish per56 litre volume. The total count of  erythrocytes and leucocytes increased until the fourth week of rearingperiod. The highest of total erythrocyte and leucocytes were observed in fish treated with 150 mg phycocyaninper kg diet ( 13.17 x  105 cells/mm3 and 8.93 x 105 cells/mm3 respectively) which were not significantlydifferent (P>0.05) to those treated with 250 mg phycocyanin per kg diet. The total leucocytes and phagocyticactivity of fish fed diet containing  250 mg phycocyanin  per kg diet (8.49 x 105 cells/mm3 and 59.67%respectively) were significantly higher  (P <0.05) to those of control group. The highest of final weight(Wt=14.32 g) and weight growth (G=5.89g) and lowest of feed conversion ratio (FCR=1.13) were obtainedin fish treated with  250 mg phycocyanin per kg diet which were  significantly  higher  (P <0.05) than thosefed control diet. The data showed that  the addition of  phycocyanin 250 mg/kg diet enhances the totalleukocyte count, phagocytic activity and the growth of humpback grouper juvenil.
Enhancement of Nonspecific Immune Response and Growth Performance of Litopenaeus vannamei by Oral Administration of Nucleotides (PENINGKATAN RESPONS IMUN NONSPESIFIK DAN PERFORMA PERTUMBUHAN LITOPENAEUS VANNAMEI MELALUI PEMBERIAN NUKLEOTIDA SECARA ORAL) Manoppo, Henky; ., Sukenda
Jurnal Veteriner Vol 14, No 4 (2013)
Publisher : Jurnal Veteriner

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This research evaluated the nonspecific immune responseand growth of Litopenaeus vannamei fednucleotide diet.  In Laboratory, juveniles were reared in two groups of glass aquaria, each with threereplications.  Shrimps in group one were fed nucleotide diet and in group two were fedpellet four consecutiveweeks. Total Haemocyte Count and Phenoleoxydase activity were evaluated at the end of feeding whilegrowth was measured at two weeks interval. At the end of feeding, shrimps were intramuscularlyinjectedwith Vibrio harveyi  0.1x106 cfu.shrimp-1.  In tambak, juveniles were raised in two groups of net cages(hapa), each with three replications. One group was fed nucleotide diet while the other wasfed pellet forfour weeks. Total Haemocyte Count of shrimp fed nucleotide diet significantly increased up to 87% higherthan shrimps fed pellet.  Phenoleoxydase activity of shrimp fed nucleotides diet also increased isignificantlyas compared to shrimp fed pellet (p=0.02). Higher resistance and growth were observed in shrimp fednucleotide diet. In tambak, weight gain of shrimp fed nucleotide was 35.75% greater than shrimp fedpellet. Survival rate (83.24%) was higher than shrimp fed pellet (81.71%).  As conclusion, oral administrationof nucleotide at 400 mg.kg-1 diet could enhancethe nonspecific immune response, resistance, and growth ofL. vannamei.
FRAKSINASI DAN UJI TOKSISITAS ECP (Extracellular Product) Streptococcus agalactiae ISOLAT NK1 PADA IKAN NILA (Oreochromis niloticus) Suryadi, Ibnu Bangkit Bioshina; ., Sukenda; Nuryati, Sri
Jurnal Perikanan Kelautan Vol 8, No 1 (2017): Jurnal Perikanan dan Kelautan Unpad
Publisher : Fakultas Perikanan dan Ilmu Kelautan Universitas Padjadjaran

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Penelitian dilaksanakan dari bulan Januari hingga September 2016 di Laboratorium Kesehatan Ikan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor. Penelitian ini bertujuan untuk mengetahui fraksi protein toksik dari ECP (Extracellular Product) dari bakteri Streptococcus agalactiae. 70 fraksi protein ECP S. aglactiae yang dihasilkan melalui metode kolom kromatografi, masing-masing fraksi disuntikkan secara intraperitonial pada lima ekor ikan nila dengan bobot rata-rata 20 g. Kemudian dilakukan analisis sodium dodecyl sulfate – polyacrilamid gel electrophoresis (SDS-PAGE) untuk mengetahui bobot molekul fraksi protein toksik. Parameter yang diamati adalah konsentrasi protein ECP S. agalactiae isolat NK1, gejala klinis dan mortalitas. Hasil penelitian menunjukkan bahwa terdapat delapan fraksi protein toksik dari ECP S. agalactiae isolat NK1 yaitu fraksi protein no. 6, 15, 18, 23, 28, 54 dan 70.
TOKSISITAS DAN IMUNOGENISITAS PRODUK EKSTRASELULER Streptococcus agalactiae TIPE NON-HEMOLITIK PADA IKAN NILA (Oreochromis niloticus ) Dwinanti, Sefti Heza; ., Sukenda; Yuhana, Munti; M Lusiastuti, Angela
Jurnal Akuakultur Rawa Indonesia Vol 2, No 1 (2014): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

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ABSTRACT The aim of this research was to analyse the toxicity and immunogenicity of ECP of non-hemolytic S. agalactiae and to evaluate ECP as a material vaccine for prevention of S. agalactiae. Toxicity to tilapia was detected in the ECP. There was a chronic  mortality pattern which was shown after post ECP injection. By intraperitoneal injection into young tilapia (± 20 g), the median lethal dose were calculated at 633,9 µg/kg bodyweight for isolate 3 and 685,4 µg/kg bodyweight for isolate 5. Cytopathic effects of ECP to tilapia’s lymphocytes were observed during 0, 30, 60 and 180 minutes. A mixture of ECP caused nuclear granulation and cytoplasmic streaming after 60 minutes. Using immunodiffusion analysis, precipitate line was demonstrated against tilapia anti-ECP sera, and there was bio-specific type anti sera against for each isolate. The ECP vaccine effective to protect tilapia from S. agalactiae was injected intraperitoneally (IP) at 2 x 105 CFU/ml. Tilapia vaccinated with ECP from isolat 3 which protein  283 µg/kg bodyweight had a RPS value 60%.  Tilapia vaccinated with ECP from isolat 5 which protein 408 µg/kg  had a RPS value 68%.   Keywords:  toxicity, immunogenicity, extracellular products, Streptococcus agalactiae, tilapia