Rugayah .
Dosen Jurusan Agroteknologi, Fakultas Pertanian Universitas Lampung, Jln. Prof. Soemantri Brodjonegoro, No. 1, Bandar Lampung 35145

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Pangium edule, an Almost Forgotten Plant and Its Potential Partomihardjo, T; ., Rugayah
MEDIA KONSERVASI Vol 2, No 2 (1989): Media Konservasi
Publisher : MEDIA KONSERVASI

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Abstract

Pangi (Pangium edule Reinw.) is one of the Indonesian plant, which is recently becoming rare, especially in Java and Sumatera. Based on the herbarium collections, the plant is distributed throughout Indonesia. It grows well below 300 m above sea level, but it is found up to 1000 m a.s.l., in primary and secondary rain forests, also a long river banks, in teak forests, on dryland, stony or clay soils. Pangi is known as a poisonous tree, but the seeds have been extensively utilized for many favorite dishes. The fresh leaves or seeds or the oil are used as an anticeptic, disinfectant or antiparasitic, to preserve meat and to kill fish, etc. The seeds seem to be dispersed by water, because they have excellent floating capacity. However, in Bacan Island, North Maluku, they might also be dispersed by black monkeys.
Genetic Relatedness among Duku, Kokosan, and Pisitan in Indonesia Based on Random Amplified Polymorphic DNA Markers Hanum, Laila; Kasiamdari, Rina Sri; ., Santosa; ., Rugayah
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

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Abstract

Genetic relatedness among duku, kokosan, and pisitan from Indonesia were investigated using random amplified polymorphic DNA (RAPD) markers. Eleven primers (OPA-01, OPA-02, OPA-10, OPB-07, OPB-11, OPB-12, OPB-15, OPT-16, OPU-14, OPU-19, and OPU-20) were used for amplification and yielded a total of 174 DNA bands, of which 167 were polymorphic. Primer OPA-10, OPB-11, OPB-12, OPB-15, and OPU-19 produced all of the polymorphic DNA bands. The size of the amplified DNA fragments ranged from 41-1546 bp. The dendrogram separated into two clusters at a genetic similarity coefficient of 0.76. The cluster 1 consisted of subclusters duku and several pisitan (pisitan OKI, pisitan Sleman, pisitan Hatu, pisitan Punggur, and pisitan Tanjung), and cluster 2 consisted of subclusters kokosan and pisitan. In the kokosan subclusters, including duku Drendan. Dendrogram supported the determination of taxonomic status of duku, kokosan, and pisitan as one species, namely Lansium domesticum Corr. and its divided into two groups, namely L. domesticum ’duku group’ and L. domesticum ’pisitan-kokosan group’. Thus, RAPD analysis was useful tool for determining the genetic variation and the genetics relatedness among duku, kokosan, and pisitan in Indonesia.Key words: duku, kokosan, pisitan/langsat, genetic relatedness, RAPD
The Phylogenetic Relationship Among Varieties of Lansium domesticum Correa Based on ITS rDNA Sequences Hanum, Laila; Kasiamdari, Rina Sri; ., Santosa; ., Rugayah
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

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Abstract

Lansium domesticum Corr. with vernacular name in Indonesian duku has been reported containingtherapeutic bioactive compounds, and some of these compounds shown to be potent antitumor, anticancer,antimalaria, antimelanogenesis, antibacteria, and antimutagenic activities. This plant is commonly known asduku, kokosan and langsat by the local community in Indonesia. The morphological appearance of all varieties isnearly the same, and identifi cation of the varieties is very diffi cult for growers. Variation of DNA sequences ofthe ITS (Internal transcribed spacer) region can be used as a molecular character to determine the phylogeneticrelationship of different varieties of L. domesticum. The aims of this study were to determine taxonomy status ofduku, kokosan, and langsat, also phylogenetic relationship among varieties of L. domesticum based on ITS rDNAsequencing. DNA was isolated from leaves of plant and then amplifi ed using F1 and R1 primers. Nucleotidesequences were identifi ed using Sequence Scanner Software Programm version 1.0, nucleotide sequences from18S, ITS1, 5.8S, ITS2 and 26S region, that has been mergered using EditSeq and SegMan in software Suite forSequence Analysis DNASTAR Lasergene DM version 3.0.25. The results of study showed that DNA fragmentsranging in size from 782-810 bp. Different pattern of DNA fragments indicated polymorphism among duku,kokosan, and langsat. Based on the results of the ITS rDNA sequencing and phylogenetic tree analysis. Itwas determined that Lansium and Aglaia are a separated genus with the similarity index value of 0.98. Duku,kokosan and langsat were divided into two cluster, namely cluster kokosan-langsat and cluster duku with thesimilarity index value of 0.996.Keywords : Phylogenetic relationship, ITS region, L. domesticum, duku, kokosan, langsat
Pangium edule, an Almost Forgotten Plant and Its Potential Partomihardjo, T; ., Rugayah
Media Konservasi Vol 2, No 2 (1989): Media Konservasi
Publisher : Deparement of Forest Resources Conservation and Ecotourism - Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (523.963 KB) | DOI: 10.29244/medkon.2.2.%p

Abstract

Pangi (Pangium edule Reinw.) is one of the Indonesian plant, which is recently becoming rare, especially in Java and Sumatera. Based on the herbarium collections, the plant is distributed throughout Indonesia. It grows well below 300 m above sea level, but it is found up to 1000 m a.s.l., in primary and secondary rain forests, also a long river banks, in teak forests, on dryland, stony or clay soils. Pangi is known as a poisonous tree, but the seeds have been extensively utilized for many favorite dishes. The fresh leaves or seeds or the oil are used as an anticeptic, disinfectant or antiparasitic, to preserve meat and to kill fish, etc. The seeds seem to be dispersed by water, because they have excellent floating capacity. However, in Bacan Island, North Maluku, they might also be dispersed by black monkeys.
Freycinetia of Mount Nyiut and Palung, West Kalimantan based on Leaf Anatomical Characters Rizki, Fitri Sri; Chikmawati, Tatik; ., Rugayah
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2688.694 KB) | DOI: 10.14203/jbi.v11i1.2165

Abstract

Freycinetia Gaudich. is a climber plant belongs to the family of Pandanaceae. Seven species of Freycinetia from Mount of Nyiut and Palung located in West Kalimantan have been recognized based on leaf anatomy. Paradermal and transversal leaf sections of those species were observed, and the data was used to construct a dendrogram using Jaccard similarity index  and Unweighted Pair Group Method with Arithmetic Average (UPGMA) method. Six main characters have been used to distingush and evaluate the similarity between its, i.e. number of hypodermal layers, number of palisade layers, shape of sponge tissue, layout of sclerenchyma tissue, stomata size,  presence of costal and intercostal cells, and shape of vascular bundle. Cluster analysis based on the anatomical data showed that seven Freycinetia species were clustered into two major groups at similarity coefficient 0.38. The first group consisted of F. angustifolia,   F. imbricata, F. sarawakensis, and F. winkleriana, while the second group consisted of F. corneri, F. sessiliflora and F. sumatrana. Keywords: Anatomical variation, Freycinetia, West Kalimantan