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Journal : Jurnal Ilmu Ternak dan Veteriner

Post-partum reproductive appearance of buffalo at various levels of GnRH and synchronized with PGF ., Yendraliza; Zespin, B.P; Udin, Z; ., Jaswandi
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 2 (2012): JUNE 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.819 KB) | DOI: 10.14334/jitv.v17i2.684

Abstract

A study of reproductive performance of buffalo treated with levels of GnRH was conducted on twenty buffaloes of Kampar. The animals were divided into five groups. Group 1 received 200 µg GnRH, the second group received 250 µg GnRH, the third group received 300 µg GnRH, the fourth group received 350 µg GnRH and the fifth group received 400 µg GnRH. Each group also received 12.5 µg PGF2α at days 7 following GnRH injection. Sign of estrus was observed 9 days after GnRH injection. Statistical analysis was performed based on completely randomized design. Results showed that the injection of 300µg of GnRH that was followed by 12,5 µg of PGF2α was able to show obvious sign of post-partum oestrus, with sign of estrus appeared at oestrus rate of 27.8 hours, following PGF2α injection, with 100% of pregnancy rate and 100% of calving rate. Key Words: Estrus, Pregnancy Rate, Calving Rate
Use of pregnant mare’s sera gonadotropin (PMSG) in media in vitro maturation of cow oocytes Udin, Zaituni; ., Jaswandi; Afriani, Tinda; E, Leonardo
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 1 (2007): MARCH 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.149 KB) | DOI: 10.14334/jitv.v12i1.564

Abstract

It is known that hormone addition in media helps in vitro maturation of oocyte. This research was aimmed to determine the effect of PMSG in media to maturation rate and nucleous developvement of cow oocyte. Ovaries were obtainned from local slaughterhouse. The media used for in vitro maturation of oocyte was TCM- 199 and the treatment was 3 levels of PMSG: 0, 10 and 20 mg/ml. Result of this research showed that the dose of PMSG in maturation media was significantly affected (P<0.05)  nucleolus development of oocytes and maturation rate. The average of germinal vesicle (GV) stage in 3 levels of PMSG 0, 10 and 20 mg/ml were 38.33; 12.64 and 9.64%, respectivelly. There was no germinal vesicle breakdown (GVBD) found in 3 levels of PMSG addition. The nucleous development of metaphase–I (M-I) were 7.64; 20.2 and 22.00%, but the average of maturation rate (M-II)  was 16.32; 48.10 and 35.34% for 3 levels of PMSG: 0, 10 and 20 mg/ml, respectivelly. It is concluded that 10 mg/ml PMSG in media of in vitro maturation resuls in the highest maturation rate of cow oocyte. Key Words: Oocyte, Maturation, In vitro, Hormone, PMSG
The Manipulation effectivity of cell co-cultures in 5% CO2 incubation system to increase in vitro cattle embryo production Syaiful, Ferry Lismanto; BP, Zesfin; Saladin, R; ., Jaswandi; ., Hendri
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 1 (2010): MARCH 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v15i1.678

Abstract

The purpose of this research is to determine the effectivity of various cell co-cultures of cattle embryo production by in vitro CO2 5% incubation system and get the best cell co-culture. Cell co-culture which are used in the synthesis is the oviduct cells, isthmus cells, ampulla cells, follicle cells and without cells. Data were analyzed based on completely randomized desiggn. The average growth rate/ cleavage in various cell culture was: the oviduct cell 59.24%, ampulla cell  58.69%, isthmus cell 58.25%, follicle cell 52.24% and without cells 47.76%. The average  growth of 8-16 cells embryos to various cell co-culture was: the oviduct cell 46.02%, ampulla cell 45.45%, isthmus cell 45.15%, follicle cell 43.07%, and without cell 38.50%. The mean percentage of morula in various cell co-culture treatment was: the oviduct cell 20.59%, ampulla cell 20.48%, isthmus cell 20.30%, follicle cell 16.96% and without cell 12.58%. The average percentage of embryonic growth (cleavage, 8-16 cells and morula) was not significantly different (P > 0.05).  The treatment of a variety of cell co-culture increased significantly (P>0.05), blastocysts production, namely: the oviduct cell 3.28%, ampulla cell 3.22%, isthmus cell 3.08%, follicle cell 2.45% and without cell 1.97%.  In conclusion, the treatment of various cell co-culture in 5%CO2   incubation system can increace the growth of cattle embryos in vitro.   Key words: Cell Co-Culture, In Vitro Embryo, 5%CO2 Incubation System, Cattle