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Post-partum reproductive appearance of buffalo at various levels of GnRH and synchronized with PGF ., Yendraliza; Zespin, B.P; Udin, Z; ., Jaswandi
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 2 (2012): JUNE 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.819 KB) | DOI: 10.14334/jitv.v17i2.684

Abstract

A study of reproductive performance of buffalo treated with levels of GnRH was conducted on twenty buffaloes of Kampar. The animals were divided into five groups. Group 1 received 200 µg GnRH, the second group received 250 µg GnRH, the third group received 300 µg GnRH, the fourth group received 350 µg GnRH and the fifth group received 400 µg GnRH. Each group also received 12.5 µg PGF2α at days 7 following GnRH injection. Sign of estrus was observed 9 days after GnRH injection. Statistical analysis was performed based on completely randomized design. Results showed that the injection of 300µg of GnRH that was followed by 12,5 µg of PGF2α was able to show obvious sign of post-partum oestrus, with sign of estrus appeared at oestrus rate of 27.8 hours, following PGF2α injection, with 100% of pregnancy rate and 100% of calving rate. Key Words: Estrus, Pregnancy Rate, Calving Rate
Use of pregnant mare’s sera gonadotropin (PMSG) in media in vitro maturation of cow oocytes Udin, Zaituni; ., Jaswandi; Afriani, Tinda; E, Leonardo
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 1 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v12i1.564

Abstract

It is known that hormone addition in media helps in vitro maturation of oocyte. This research was aimmed to determine the effect of PMSG in media to maturation rate and nucleous developvement of cow oocyte. Ovaries were obtainned from local slaughterhouse. The media used for in vitro maturation of oocyte was TCM- 199 and the treatment was 3 levels of PMSG: 0, 10 and 20 mg/ml. Result of this research showed that the dose of PMSG in maturation media was significantly affected (P<0.05)  nucleolus development of oocytes and maturation rate. The average of germinal vesicle (GV) stage in 3 levels of PMSG 0, 10 and 20 mg/ml were 38.33; 12.64 and 9.64%, respectivelly. There was no germinal vesicle breakdown (GVBD) found in 3 levels of PMSG addition. The nucleous development of metaphase–I (M-I) were 7.64; 20.2 and 22.00%, but the average of maturation rate (M-II)  was 16.32; 48.10 and 35.34% for 3 levels of PMSG: 0, 10 and 20 mg/ml, respectivelly. It is concluded that 10 mg/ml PMSG in media of in vitro maturation resuls in the highest maturation rate of cow oocyte. Key Words: Oocyte, Maturation, In vitro, Hormone, PMSG
The Manipulation effectivity of cell co-cultures in 5% CO2 incubation system to increase in vitro cattle embryo production Syaiful, Ferry Lismanto; BP, Zesfin; Saladin, R; ., Jaswandi; ., Hendri
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 1 (2010)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v15i1.678

Abstract

The purpose of this research is to determine the effectivity of various cell co-cultures of cattle embryo production by in vitro CO2 5% incubation system and get the best cell co-culture. Cell co-culture which are used in the synthesis is the oviduct cells, isthmus cells, ampulla cells, follicle cells and without cells. Data were analyzed based on completely randomized desiggn. The average growth rate/ cleavage in various cell culture was: the oviduct cell 59.24%, ampulla cell  58.69%, isthmus cell 58.25%, follicle cell 52.24% and without cells 47.76%. The average  growth of 8-16 cells embryos to various cell co-culture was: the oviduct cell 46.02%, ampulla cell 45.45%, isthmus cell 45.15%, follicle cell 43.07%, and without cell 38.50%. The mean percentage of morula in various cell co-culture treatment was: the oviduct cell 20.59%, ampulla cell 20.48%, isthmus cell 20.30%, follicle cell 16.96% and without cell 12.58%. The average percentage of embryonic growth (cleavage, 8-16 cells and morula) was not significantly different (P > 0.05).  The treatment of a variety of cell co-culture increased significantly (P>0.05), blastocysts production, namely: the oviduct cell 3.28%, ampulla cell 3.22%, isthmus cell 3.08%, follicle cell 2.45% and without cell 1.97%.  In conclusion, the treatment of various cell co-culture in 5%CO2   incubation system can increace the growth of cattle embryos in vitro.   Key words: Cell Co-Culture, In Vitro Embryo, 5%CO2 Incubation System, Cattle
Post-partum reproductive appearance of buffalo at various levels of GnRH and synchronized with PGF ., Yendraliza; Zespin, B.P; Udin, Z; ., Jaswandi
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 2 (2012)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v17i2.684

Abstract

A study of reproductive performance of buffalo treated with levels of GnRH was conducted on twenty buffaloes of Kampar. The animals were divided into five groups. Group 1 received 200 µg GnRH, the second group received 250 µg GnRH, the third group received 300 µg GnRH, the fourth group received 350 µg GnRH and the fifth group received 400 µg GnRH. Each group also received 12.5 µg PGF2α at days 7 following GnRH injection. Sign of estrus was observed 9 days after GnRH injection. Statistical analysis was performed based on completely randomized design. Results showed that the injection of 300µg of GnRH that was followed by 12,5 µg of PGF2α was able to show obvious sign of post-partum oestrus, with sign of estrus appeared at oestrus rate of 27.8 hours, following PGF2α injection, with 100% of pregnancy rate and 100% of calving rate. Key Words: Estrus, Pregnancy Rate, Calving Rate
The Manipulation effectivity of cell co-cultures in 5% CO2 incubation system to increase in vitro cattle embryo production Syaiful, Ferry Lismanto; BP, Zesfin; Saladin, R; ., Jaswandi; ., Hendri
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 1 (2010): MARCH 2010
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.437 KB) | DOI: 10.14334/jitv.v15i1.678

Abstract

The purpose of this research is to determine the effectivity of various cell co-cultures of cattle embryo production by in vitro CO2 5% incubation system and get the best cell co-culture. Cell co-culture which are used in the synthesis is the oviduct cells, isthmus cells, ampulla cells, follicle cells and without cells. Data were analyzed based on completely randomized desiggn. The average growth rate/ cleavage in various cell culture was: the oviduct cell 59.24%, ampulla cell  58.69%, isthmus cell 58.25%, follicle cell 52.24% and without cells 47.76%. The average  growth of 8-16 cells embryos to various cell co-culture was: the oviduct cell 46.02%, ampulla cell 45.45%, isthmus cell 45.15%, follicle cell 43.07%, and without cell 38.50%. The mean percentage of morula in various cell co-culture treatment was: the oviduct cell 20.59%, ampulla cell 20.48%, isthmus cell 20.30%, follicle cell 16.96% and without cell 12.58%. The average percentage of embryonic growth (cleavage, 8-16 cells and morula) was not significantly different (P > 0.05).  The treatment of a variety of cell co-culture increased significantly (P>0.05), blastocysts production, namely: the oviduct cell 3.28%, ampulla cell 3.22%, isthmus cell 3.08%, follicle cell 2.45% and without cell 1.97%.  In conclusion, the treatment of various cell co-culture in 5%CO2   incubation system can increace the growth of cattle embryos in vitro.   Key words: Cell Co-Culture, In Vitro Embryo, 5%CO2 Incubation System, Cattle
Use of pregnant mare’s sera gonadotropin (PMSG) in media in vitro maturation of cow oocytes Udin, Zaituni; ., Jaswandi; Afriani, Tinda; E, Leonardo
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 1 (2007): MARCH 2007
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.149 KB) | DOI: 10.14334/jitv.v12i1.564

Abstract

It is known that hormone addition in media helps in vitro maturation of oocyte. This research was aimmed to determine the effect of PMSG in media to maturation rate and nucleous developvement of cow oocyte. Ovaries were obtainned from local slaughterhouse. The media used for in vitro maturation of oocyte was TCM- 199 and the treatment was 3 levels of PMSG: 0, 10 and 20 mg/ml. Result of this research showed that the dose of PMSG in maturation media was significantly affected (P<0.05)  nucleolus development of oocytes and maturation rate. The average of germinal vesicle (GV) stage in 3 levels of PMSG 0, 10 and 20 mg/ml were 38.33; 12.64 and 9.64%, respectivelly. There was no germinal vesicle breakdown (GVBD) found in 3 levels of PMSG addition. The nucleous development of metaphase–I (M-I) were 7.64; 20.2 and 22.00%, but the average of maturation rate (M-II)  was 16.32; 48.10 and 35.34% for 3 levels of PMSG: 0, 10 and 20 mg/ml, respectivelly. It is concluded that 10 mg/ml PMSG in media of in vitro maturation resuls in the highest maturation rate of cow oocyte. Key Words: Oocyte, Maturation, In vitro, Hormone, PMSG
Post-partum reproductive appearance of buffalo at various levels of GnRH and synchronized with PGF ., Yendraliza; Zespin, B.P; Udin, Z; ., Jaswandi
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 2 (2012): JUNE 2012
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.819 KB) | DOI: 10.14334/jitv.v17i2.684

Abstract

A study of reproductive performance of buffalo treated with levels of GnRH was conducted on twenty buffaloes of Kampar. The animals were divided into five groups. Group 1 received 200 µg GnRH, the second group received 250 µg GnRH, the third group received 300 µg GnRH, the fourth group received 350 µg GnRH and the fifth group received 400 µg GnRH. Each group also received 12.5 µg PGF2α at days 7 following GnRH injection. Sign of estrus was observed 9 days after GnRH injection. Statistical analysis was performed based on completely randomized design. Results showed that the injection of 300µg of GnRH that was followed by 12,5 µg of PGF2α was able to show obvious sign of post-partum oestrus, with sign of estrus appeared at oestrus rate of 27.8 hours, following PGF2α injection, with 100% of pregnancy rate and 100% of calving rate. Key Words: Estrus, Pregnancy Rate, Calving Rate
Sistem Informasi Digital dan Pencari Jalan Sukmawati, Lisna; ., Jaswandi; Maulana, Adidin Aidin; Rahman, Abdul
Telekontran : Jurnal Ilmiah Telekomunikasi, Kendali dan Elektronika Terapan Vol 7 No 1 (2019): TELEKONTRAN
Publisher : Program Studi Teknik Elektro, Fakultas Teknik dan Ilmu Komputer, Universitas Komputer Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (712.582 KB) | DOI: 10.34010/telekontran.v7i1.1639

Abstract

Umumnya mahasiswa baru dan pengunjung baru di sebuah gedung, sering mengalami kesulitan ketika pencarian sebuah ruang, terutama gedung yang memiliki banyak ruang. Serta memiliki ruang yang komplek, baik ruang kelas dan ruang kerja staff. Biasanya pengunjung yang baru masuk kedalam lingkungan gedung tersebut bertanya pada pihak informasi kemanan ataupun pengunjung lain untuk memberikan informasi ruang kelas atau ruang kerja staff yang dicari. Cara lain pengunjung mencari informasi ruangan konvensional gedung yang tersedia, akan tetapi cara alternatif&nbsp; tersebut membutuhkan banyak membuang waktu. Untuk itu, pada penelitian ini dibuat di gedung kampus, dengan menggunakan software NetBeans IDE menerapkan algoritma pencarian jalur terdekat berdasarkan nilai indek pixel pada gambar peta. Sistem akan menampilkan peta arahan yang terdekat dan informasi ruang staff ruang dosen dan ruang kelas kuliah dan pencarian informasi data dosen. Begitu juga pencarian staff dan dosen dengan tulis namanya pada panel pencarian di monitor touch screen. lalu menekan tombol “take me there”,, maka sistem akan menunjukan arah menuju ruang kerja dan memberikan informasi staff. Untuk melihat informasi pengumuman, pengguna cukup memilih menu pengumuman, lalu memilih kategori pengumuman. Dengan adanya sistem ini diharapkan pengunjung tidak kesulitan dalam pencarian ruang dan staff serta informasi kampus. Tujuan dari penelitian ini adalah, dapat memberikan peta arah gedung di kampus dari posisi pemasangan sistem menuju ruang kelas maupun ruang kerja staff dan ruang dosen. Selain itu juga dapat memberikan informasi seputar aktivitas kampus, pengumuman bahkan event kepada mahasiswa dan pengunjung, di rancang dengan antarmuka yang menarik&nbsp; mudah digunakan. Kata kunci : &nbsp;Peta Gedung, Waifinding, Digital Informasi, layar LCD, NetBeans IDE
Use of pregnant mare’s sera gonadotropin (PMSG) in media in vitro maturation of cow oocytes Udin, Zaituni; ., Jaswandi; Afriani, Tinda; E, Leonardo
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 1 (2007): MARCH 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.149 KB) | DOI: 10.14334/jitv.v12i1.564

Abstract

It is known that hormone addition in media helps in vitro maturation of oocyte. This research was aimmed to determine the effect of PMSG in media to maturation rate and nucleous developvement of cow oocyte. Ovaries were obtainned from local slaughterhouse. The media used for in vitro maturation of oocyte was TCM- 199 and the treatment was 3 levels of PMSG: 0, 10 and 20 mg/ml. Result of this research showed that the dose of PMSG in maturation media was significantly affected (P&lt;0.05)  nucleolus development of oocytes and maturation rate. The average of germinal vesicle (GV) stage in 3 levels of PMSG 0, 10 and 20 mg/ml were 38.33; 12.64 and 9.64%, respectivelly. There was no germinal vesicle breakdown (GVBD) found in 3 levels of PMSG addition. The nucleous development of metaphase–I (M-I) were 7.64; 20.2 and 22.00%, but the average of maturation rate (M-II)  was 16.32; 48.10 and 35.34% for 3 levels of PMSG: 0, 10 and 20 mg/ml, respectivelly. It is concluded that 10 mg/ml PMSG in media of in vitro maturation resuls in the highest maturation rate of cow oocyte. Key Words: Oocyte, Maturation, In vitro, Hormone, PMSG