Hamny .
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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Karakteristik histologi perkembangan folikel ovariumfase luteal pada kancil (Tragulus javanicus)

Hemera Zoa Vol 2, No 1 (2010): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

The lesser mouse-deer (Tragulus javanicus) is one of the Indonesia biodiversity. This animal distributes only in South-East Asia and Hindia. The lesser mouse-deer is the smallest ungulate in the world and regarded as an ideal model to biomedic and ruminant research. As their population becoming extincted, conservation efSort is very important. One of the eflorts in supporting the lesser mouse-deer conservation is by improving our knowledge reproductive physiology of the female lesser mouse-deer. The aim of this study was to investigate the histological characteristic of ovarian follicles development of the female lesser mouse-deer. Experiment was done on the ovary (n=4). The ovary was fixed in Bouin solution andprocessed according to standard of histology. The slides were stained in HE. At luteal phase, the developing follicles can be classified into 10 stages. The number of developing follicles in the left ovary were higher then in the right.  

Sebaran Karbohidrat pada Kelenjar Ludah Biawak Air (Varanus salvator) (DISTRIBUTION OF CARBOHYDRATES IN THE SALIVARY GLANDS OF WATER MONITOR (VARANUS SALVATOR))

Jurnal Veteriner Vol 15, No 4 (2014)
Publisher : Jurnal Veteriner

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Abstract

The objective of this study was to determine the carbohydrate content of salivary glands lingual,sublingual, and mandibular glands of water monitor (Varanus salvator). This studywas used the lingualglands, sublingual, and mandibularof an adult male water monitor. Salivary glands were fixed by soakingin a solution of 4% paraformaldehyde for four days. Then, the tissuewas processed into routine histologicalpreparations. Salivary gland tissue was stained with alcianblue staining (AB) pH 2.5 for detecting acidiccarbohydrates and was stained with periodic acid Schiff (PAS) for detecting neutral carbohydrates. Theresults showed that the lingual and sublingual glands showed a positive reaction to AB pH 2.5 stainingand PAS with the intensity varied from weak (+) to strong intensity (+++) scattered in the cytoplasm of thecell. In the cytoplasm of cells of the mandibular glands showed negative reaction (-) to the AB pH 2.5staining but reacted positively to PAS with the intensity varied from weak (+) to strong (+++). Based onthe result of the study it can be concluded that lingual and sublingual glands of Water monitor containacidic and neutral carbohydrates while mandibular glands only contain neutral carbohydrates.

Morfologi Anatomi dan Histologi Usus Biawak Air (Varanus salvator) THE ANATOMICAL AND HISTOLOGICAL MORPHOLOGY OF INTESTINAL WATER MONITOR (VARANUS SALVATOR)

Jurnal Veteriner Vol 16, No 2 (2015)
Publisher : Jurnal Veteriner

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Abstract

The objective of this study was to determine the anatomical morphology and histology of the intestineof water monitor (Varanussalvator). Intestine organ from an adult male water monito was used in thisstudy. Perfusion was performed to flow the 4% paraformaldehyde fixative solution through the heart.Intestine was collected, then divided into six sections, followed with dipping them into 4% paraformaldehydesolution for four days, continued histological preparations and stained with haematoxylin eosin (HE). Theanatomical morphology observation was performed before the intestine was processed into histologicalpreparations by observing the outer shape of the intestine and measuring it’slength. Morphologicalobservation was made †after processing theintestinal tissue by observing each portion of the intestinalwall structure. The results showed that transverse fold were found on the outer and inner surfaces of theintestine section I-IV, while in the V-VI part of the intestine, the folds were not observed anymore. Theintestine wall consisted of four layers, such as mucosa, submucosa, tunica muscularis, and serosa. Thevilli on intestine section I-IV were relatively longer and higher, while the intestine vili in section V-VI wererelatively short. The entire mucosa in water monitor was coated with absorptive columnar epitheliumwith goblet cells. Based on the result study, it can be concluded that the water monitor has more efficientdigestive process which can be seen morphologically from the relatively short intestinal, has transversefolds on the surface of the small intestine, and does not have cecum. The intestinal wall consists of layersi.e : mucosa, submucosa, tunica muscularis, and serosa.