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Optimasi transplantasi menggunakan sel donor dari ikan gurame muda dan ikan nila triploid sebagai resipien ., Alimuddin; Junior, M. Zairin; Arfah, Harton
Jurnal Ilmu Pertanian Indonesia Vol 15, No 3 (2010): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Testicular cell transplantation technology can be used in fish seed production engineering. In this study, optimization of transplantation using donor cells from young gouramy and triploid tilapia (3N) as recipient. Triploid tilapia is produced using heat shock method. The testes of male gouramy (body weight of 400- 850 g) was dissociated using 0.5% trypsin. Dissociated testicular cells was injected into the peritoneal cavity of tilapia larvae. Analysis of donor cell colonization was carried out using PCR method with DNA template that had been extracted from the gonad of 2-month-old tilapia. PCR was performed using specific primers for the growthhormone gene and (3-actin as an internal control of DNA loading. The results of nucleoli preparation showed thatthe success of triploidyzation was 88.5%. The gonad size of diploid (2N) and 3N recipient were relatively similar, while in not transplanted 3N tilapia was rudimentary. PCR results showed that the transplanted 3N tilapia has a DNA band of the same size with gouramy, while in control was not. This indicated that donor cells have been colonized in the gonads of recipient. The donor cell colonization in recipient 3N (78%) was higher than that of 2N (50%). Further research is required to determine the ability of donor cells differentiate into sperm and eggs in recipient gonad.Keywords : Spermatogonia, cell transplantation, triploid, giant gouramy.
Fermentation quality and nutritive value of rice crop residue based silage ensiled with addition of epiphytic lactic acid bacteria Santoso, B; Hariadi, B.Tj.; ., Alimuddin; Seseray, D.Y.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 1 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v16i1.628

Abstract

Silage is the feedstuff resulted from the preservation of forages through lactic acid fermentation. The aim of this study was to evaluate nutritive value, fermentation characteristics and nutrients digestibility of rice crop residue based silage ensiled with epiphytic lactic acid bacteria (LAB). The mixture of rice crop residue (RC), soybean curd residue (SC) and cassava waste (CW) in a 90: 5: 5 (on dry matter basis) ratio was used as silage material.  Three treatments silage were (A) RC + SC + CW as a control; (B) RC + SC + CW + LAB inoculums from rice crop residue; (C) RC + SC + CW + LAB inoculums from king grass.  Silage materials were packed into plastic silo (1.5 kg capacity) and stored for 30 days. The results showed that crude protein content in B and C silage was higher than that of silage A, but NDF content in silages B and C was lower than that of silage A.  Lactic acid concentration was higher (P < 0.01) in silage C compared to silage B and A, thus pH value of silage C was lower (P < 0.01) than silage B and A. Silage C had the highest Fleigh point than that of other silages. Dry matter and organic matter digestibilities were higher in silages B and C (P < 0.01) than that of control silage. It was concluded that the addition of LAB inoculums from king grass to rice crop residue based silage resulted a better fermentation quality compared to LAB inoculums from rice crop residue. Key Words: Silage, Rice Crop Residue, Lactic Acid, In Vitro
Optimasi transplantasi menggunakan sel donor dari ikan gurame muda dan ikan nila triploid sebagai resipien ., Alimuddin; Junior, M. Zairin; Arfah, Harton
Jurnal Ilmu Pertanian Indonesia Vol 15, No 3 (2010): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1375.475 KB)

Abstract

Testicular cell transplantation technology can be used in fish seed production engineering. In this study, optimization of transplantation using donor cells from young gouramy and triploid tilapia (3N) as recipient. Triploid tilapia is produced using heat shock method. The testes of male gouramy (body weight of 400- 850 g) was dissociated using 0.5% trypsin. Dissociated testicular cells was injected into the peritoneal cavity of tilapia larvae. Analysis of donor cell colonization was carried out using PCR method with DNA template that had been extracted from the gonad of 2-month-old tilapia. PCR was performed using specific primers for the growth hormone gene and (3-actin as an internal control of DNA loading. The results of nucleoli preparation showed that the success of triploidyzation was 88.5%. The gonad size of diploid (2N) and 3N recipient were relatively similar, while in not transplanted 3N tilapia was rudimentary. PCR results showed that the transplanted 3N tilapia has a DNA band of the same size with gouramy, while in control was not. This indicated that donor cells have been colonized in the gonads of recipient. The donor cell colonization in recipient 3N (78%) was higher than that of 2N (50%). Further research is required to determine the ability of donor cells differentiate into sperm and eggs in recipient gonad.
An Actinomycetes Producing Anticandida Isolated from Cajuput Rhizosphere: Partial Identification of Isolates and Amplification of pks-I genes ., Alimuddin; Asmara, Widya; Widada, Jaka; ., Mustofa; Nurjasmi, Reni
Indonesian Journal of Biotechnology Vol 15, No 1 (2010)
Publisher : Universitas Gadjah Mada

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Abstract

Actinomycetes have been the most prolific producer of various kinds of antifungal metabolites, and many of them are described as being produced by polyketide synthetases (pks). We present strain of Actinomycetes producing anticandida isolated from rhizosphere plant for amplification of Pks-I genes. The isolate was obtained from Wanagama I Forest UGM Yogyakarta. Gene of seven isolates, from total of 173 isolates, were amplified using degenerate primer to detect the presence of pks genes. One strain that is named Streptomyces sp. GMR-22 was partialy identified as anticandida producing actinomycete. The strain shown the strongest activity against Candida albicans. Based on bioautography assay, one spot active with Rf 0.57 was appeared as bright yellow by cerrium sulphate but it was and not visible on UV254 and 366 lights. Key words : pks genes, anticandida, Streptomyces sp GMR-22, rep-PCR, cajuput rhizosphere
Fermentation quality and nutritive value of rice crop residue based silage ensiled with addition of epiphytic lactic acid bacteria Santoso, B; Hariadi, B.Tj.; ., Alimuddin; Seseray, D.Y.
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 1 (2011): MARCH 2011
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (254.211 KB) | DOI: 10.14334/jitv.v16i1.628

Abstract

Silage is the feedstuff resulted from the preservation of forages through lactic acid fermentation. The aim of this study was to evaluate nutritive value, fermentation characteristics and nutrients digestibility of rice crop residue based silage ensiled with epiphytic lactic acid bacteria (LAB). The mixture of rice crop residue (RC), soybean curd residue (SC) and cassava waste (CW) in a 90: 5: 5 (on dry matter basis) ratio was used as silage material.  Three treatments silage were (A) RC + SC + CW as a control; (B) RC + SC + CW + LAB inoculums from rice crop residue; (C) RC + SC + CW + LAB inoculums from king grass.  Silage materials were packed into plastic silo (1.5 kg capacity) and stored for 30 days. The results showed that crude protein content in B and C silage was higher than that of silage A, but NDF content in silages B and C was lower than that of silage A.  Lactic acid concentration was higher (P < 0.01) in silage C compared to silage B and A, thus pH value of silage C was lower (P < 0.01) than silage B and A. Silage C had the highest Fleigh point than that of other silages. Dry matter and organic matter digestibilities were higher in silages B and C (P < 0.01) than that of control silage. It was concluded that the addition of LAB inoculums from king grass to rice crop residue based silage resulted a better fermentation quality compared to LAB inoculums from rice crop residue. Key Words: Silage, Rice Crop Residue, Lactic Acid, In Vitro
Fermentation quality and nutritive value of rice crop residue based silage ensiled with addition of epiphytic lactic acid bacteria Santoso, B; Hariadi, B.Tj.; ., Alimuddin; Seseray, D.Y.
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 1 (2011): MARCH 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (254.211 KB) | DOI: 10.14334/jitv.v16i1.628

Abstract

Silage is the feedstuff resulted from the preservation of forages through lactic acid fermentation. The aim of this study was to evaluate nutritive value, fermentation characteristics and nutrients digestibility of rice crop residue based silage ensiled with epiphytic lactic acid bacteria (LAB). The mixture of rice crop residue (RC), soybean curd residue (SC) and cassava waste (CW) in a 90: 5: 5 (on dry matter basis) ratio was used as silage material.  Three treatments silage were (A) RC + SC + CW as a control; (B) RC + SC + CW + LAB inoculums from rice crop residue; (C) RC + SC + CW + LAB inoculums from king grass.  Silage materials were packed into plastic silo (1.5 kg capacity) and stored for 30 days. The results showed that crude protein content in B and C silage was higher than that of silage A, but NDF content in silages B and C was lower than that of silage A.  Lactic acid concentration was higher (P &lt; 0.01) in silage C compared to silage B and A, thus pH value of silage C was lower (P &lt; 0.01) than silage B and A. Silage C had the highest Fleigh point than that of other silages. Dry matter and organic matter digestibilities were higher in silages B and C (P &lt; 0.01) than that of control silage. It was concluded that the addition of LAB inoculums from king grass to rice crop residue based silage resulted a better fermentation quality compared to LAB inoculums from rice crop residue. Key Words: Silage, Rice Crop Residue, Lactic Acid, In Vitro