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Kajian In vitro Aktivitas Sel-Sel Trofoblas Blastosis Mencit Aging dan Pengaruhnya terhadap Kegagalan Implantasi Djuwita, Ita; Helmita, Roza; Winarto, Adi; -, Wahyudin
Jurnal Veteriner Vol 10, No 1 (2009)
Publisher : Jurnal Veteriner

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Abstract

The objectives of this in vitro study were to investigate the hatching rate, the outgrowth diameter andthe activity of mitochondria Nicotiamide Adenin Dinucleotide Dyhidrogenage (NADH)-CoQ reductase ofblastocysts trophoblast cells from aging mice. Blastocysts of aging (age >12 months) and young productive(age 2 months) mice were collected from the cornua utery at day-4 of pregnancy and were cultured inmDMEM medium supplemented with 10% New Born Calf Serum (NBCS), 10% ITS, and 50 ?g/mlgentamicine, in 5% CO2 incubator at 37°C for 10 days. The blastocysts hatching rate and the trophoblastsmonolayer were examined for their diameter outgrowth and the NADH-CoQ reductase activity. The resultsshowed that the hatching rate, the trophoblast outgrowth diameter and the activity of NADH-CoQ reductaseof blastocysts collected from productive mice were significantly higher than those collected from the agingmice (P<0,05). It can be concluded that the impairment of blastocysts implantation especially, in agingmice were caused by the low activity of the NADH-CoQ reductase that play important role in energyproduction needed for the hatching and trophoblast outgrowth.
CORE STABILITY EXCERCISE LEBIH BAIK DIBANDINGKAN MCKENZIE EXCERCISE DALAM PENURUNAN DISABILITAS PASIEN NON-SPECIFIC LOW BACK PAIN Dinda Kurniawan, Ganesa Puput; Muliarta, I Made; -, Sugijanto; Wirawan, I Made Ady; Purnawati, Susy; -, Wahyudin
Sport and Fitness Journal Volume 5, No.3, 2017
Publisher : Program Studi Magister Fisiologi Keolahragaan, Fakultas Kedokteran, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/spj.2017.v05.i03.p05

Abstract

Non-specific low back pain is the symptom of lower back pain that occurs without an obvious cause, the diagnosis is based on exclusion of specific pathology. Non-specific low back pain can result in pain, muscle spasm and muscle imbalance, it can decrease the stability of the abdominals and lower back, limitation in lumbar mobility , changes posture, and it’s couse make disability in patients with non-specific low back pain. Exercise therapy for non-specific low back pain is high recommend to increased stability and correct posture of the spine, for this case thsth can be used for exercise therapy is like McKenzie exercises and core stability exercise. The purpose of this study is to determine the core stability excercise better than McKenzie excercise for release in reduce patient disability in non-specific low back pain. This research applied experimental research method with Pre and Post Test Control Group Design. The research was conducted in Dr Soeradji Tirtonegoro Klaten. There were 32 subjects taken for this research. Disability was measured by oswestry disability index (ODI) before and after treatment. They were divided into two treatment groups consisting of core stability 16 subject for the Mckenzie exercise is 16 and the frequens are 2 times a weak in a month. Statistical test results obtained, have a decline ODI score at the first group have done with a value of p = 0.000 and the second gorup with p = 0.000. it means that both of the group are significantly improve functional activity. From the comparative test data by t-test using the data difference in both groups p value <0.05, which means indicated that there is a significant difference. Therefore, the conclusion of this research indicated thar the core stability excercise better than McKenzie excercise for release in reduce patient disability in non-specific low back pain. The study is expected to benefit in patients with non-specific low back pain in reducing disability.