Nuraeni, Hanny Siti
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Interleukin-13, Interleukin-10, Interferon-gamma and IDO Production in Response to Home Dust Mites in Allergic Asthma Kwarta, Cityta Putri; Wibowo, Heri; Khaedir, Yordan; Rengganis, Iris; Nuraeni, Hanny Siti
The Indonesian Biomedical Journal Vol 11, No 2 (2019)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v11i2.714

Abstract

BACKGROUND: Allergic asthma is a degenerative atopic disease caused by allergic or hypersensitivity type-1. More than 50% of people with allergic asthma are caused by the presence of house dust mites (HDMs) allergens.METHODS: The cellular immunity response was evaluated through a peripheral blood mononuclear cell (PBMC) culture isolated from blood, using the ficoll gradient technique. Subjects were atopic asthma groups and non-atopic asthma groups. PBMC from each subject cultured was stimulated with HDMs allergen, then incubated in a CO2 5% incubator, 37o C for 72 hours. With the multiplex assay method, interferon (IFN)-?, interleukin (IL)-13 and IL-10 were measured, meanwhile indoleamine 2,3-dioxygenase level (IDO) was measured by the enzyme-linked immunosorbent assay (ELISA) sandwich methods.RESULTS: The IFN-? production in the supernatant of PBMC cultures was stimulated by phytohemagglutinin (PHA), Roswell Park Memorial Institute (RPMI) medium and allergens. The IFN-? production in allergen-stimulated supernatants showed higher level of IFN-? in the nonatopic group (4,681,455±3,434,851) than atopic group (4,363,300±2,067,941) even though it was not statistically significant (p=0.078). There were no differences between the mean of IL-13 production in atopic asthma group and non-atopic group. The IL-10 production in allergenstimulated supernatants was shown to be higher in nonatopic group and were statistically significantly different (p=0.015). The IDO production in allergen-stimulated supernatants was shown to be higher in the non-atopic group (272,231±269,564) than in the actopic group (13,273±400), and it was significantly different (p=0.007).CONCLUSION: Cellular immune profile of subjects with allergic asthma to Dermatophagoides pterronyssinus (Der p) is characterized by a type-2 inflammatory response that is dominant compared to type-1 inflammation (higher IL-13 ratio compared to IFN-?) and to the role of anti-inflammation (higher IL-13 ratio compared to IL-10). The decline in IDO production in allergic asthma subjects to Der p is thought to be related to the low cellular immune response in expressing IFN-? compared to IL-13.KEYWORDS: interleukin-13, interleukin-10, IDO, PBMC, asthma
UJI DAYA HAMBAT AIR PERASAN DAUN KATUK TERHADAP PERTUMBUHAN BAKTERI STREPTOCOCCUS PYOGENES SECARA INVITRO Ahmad, Ahmad; Nuraeni, Hanny Siti
Jurnal Medikes (Media Informasi Kesehatan) Vol 2 No 1 (2015): April
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (451.695 KB)

Abstract

Penggunaan tanaman obat didasarkan pada kepercayaan dan pengalaman yang diturunkan dari generasi ke generasi yang bersifat pengetahuan tradisional. Daun katuk (Sauropus androgunus) merupakan salah satu tanaman obat yang bersifat sebagai antikuman dan antiprotozoa karena mengandung zat aktif diantaranya flavonoid dan tanin. Telah dilakukan penelitian mengenai daya hambat air perasan daun katuk (Sauropus androgunus) terhadap bakteri Streptococcus pyogenes dengan variasi konsentrasi dan waktu kontak tertentu. Tujuan penelitian ini untuk mengetahui berapa konsentrasi terendah air perasan daun katuk dalam menghambat Streptococcus pyogenes. Metode dalam penelitian ini adalah dilusi, yaitu variasi konsentrasi air perasan daun katuk sebanyak 5 mL dikontakkan dengan strain murni Streptococcus pyogenes sebanyak 0,1 mL dengan kerapatan 1,5x108/mL dengan variasi waktu kontak yang berbeda kemudian digores pada media Agar Darah (AD). Pengamatan dilakukan dengan melihat ada tidaknya pertumbuhan bakteri Streptococcus pyogenes pada media AD. Uji penelitian menggunakan air perasan daun katuk dengan variasi konsentrasi 25%,50%, 75%, dan 100% dengan waktu kontak 30, 60 dan 90 menit. Hasil Uji Penelitian pada air perasan daun katuk adalah hasilnya tidak dapat menghambat bakteri Streptococcus pyogenes pada konsentrasi 25%,50%, 75%, dan 100% dengan waktu kontak 30, 60 dan 90 menit. Berdasarkan hal tersebut, maka uji penelitian menggunakan air perasan daun katuk dengan variasi konsentrasi 25%,50%, 75%, dan 100% dengan waktu kontak 30, 60 dan 90 menit. Kesimpulan penelitian ini adah air perasan daun katuk tidak menghambat Streptococcus pyogenes.