Sahidin, Idin
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KARAKTERISASI SENYAWA METABOLIT SEKUNDER DAN UJI ANTI BAKTERI SPONS Phyllospongia sp. DI PERAIRAN YANG BERBEDA Saputra, Adi; Sadarun, Baru; Sahidin, Idin
Jurnal Sapa Laut (Jurnal Ilmu Kelautan) Vol 4, No 4 (2019): SAPA LAUT
Publisher : Fakultas Perikanan dan Ilmu Kelautan

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Abstract

Pembentukan senyawa metabolit sekunder dipengaruhi oleh faktor lingkungan. Jenis senyawa yang sama pada lingkungan yang berbeda akan menghasilkan metabolit yang berbeda. Penelitian ini dilaksanakan di Perairan Tanjung Tiram yang merupakan perairan terbuka dan di Lalanu yang mewakili perairan semi tertutup. Keberadaan spons jenis Phyllospongia sp yang mampu hidup di perairan tersebut menunjukkan adanya potensi kandungan metabolit sekunder. Penelitian ini bertujuan untuk mengetahui perbandingan kandungan senyawa metabolit sekunder dari kedua perairan tersebut. Metode yang digunakan adalah ekstraksi, uji KLT (Kromatografi Lapis Tipis) fitokimia dan difusi untuk mengevaluasi antibakteri. Hasil penelitian ini menunjukkan bahwa telah teridentifikasi 3 macam senyawa bioaktif yakni alkaloid, steroid/terpenoid, Flavanoid/venolik. Kandungan metabolit sekunder tertinggi terutama Steroid/Terpenoid dan Fenolik/Flafanoid dari ektrak Phyllospongia sp berasal dari perairan tertutup dengan nilai RF 0,65 dan RF 0,625. Ektrak Phyllospongia sp berpotensi sebagai antibakteri aktif terhadap bakteri Staphylococcus aureus pada konsentrasi 200 ppm dengan diameter zona hambat sebesar 10,2 mm.Kata kunci : Phyllospongia sp., perairan terbuka, perairan tertutup, metabolit sekunder, antibakteri.
Antioxidant Activity of Ethanol Extract of Polygonum pulchrum Blume Sadino, Asman; Sahidin, Idin; Wahyuni, Wahyuni
Pharmacology and Clinical Pharmacy Research Vol 1, No 2
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (377.767 KB) | DOI: 10.15416/pcpr.v1i2.15201

Abstract

The use of antioxidants from natural resources has attracted increasing interest. One of theplant that was empirically used as an antioxidant dietary supplement was Polygonum pulchrumBlume (P. pulchrum Blume). This study aimed to investigate antioxidant activityof roots, stems, leaves and flowers ethanol extract of P. pulchrum Blume. The extract wasobtained by maceration method using ethanol solvent. Antioxidant activity was determinedwith 1,1-diphenyl-picrylhydrazyl (DPPH) method. We found that ethanol extracts of P. pulchrumBlume roots and stems had strong antioxidant activity with IC50 values of 25.2 mg/land 43.26 mg/l, respectively. Ethanol extract of flowers had the weakest antioxidant activitywith IC50 value of 202.96 mg/l. Vitamin C had very strong antioxidant activity with IC50value of 3.97 mg/l. In conclusion, our study revealed that ethanol extract of Polygonumpulchrum Blume roots and stems had strong antioxidant activity. Therefore, P. pulchrumBlume might be potential as an excellent source for natural antioxidant agents for medicalapplication.Keywords: Polygonum pulchrum Blume, DPPH, antioxidant, maceration, ethanol
Acute Toxicity of Ethanol Extract of Polygonum pulchrum Blume using Brine Shrimp Lethality Test Method Sadino, Asman; Sahidin, Idin; Wahyuni, Wahyuni
Pharmacology and Clinical Pharmacy Research Vol 2, No 2
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (306.058 KB) | DOI: 10.15416/pcpr.v2i2.15210

Abstract

The potential toxicity effect presents in the medicinal plants is important to be identified forthe safety assurance. Accute toxicity study is an initial step in the drug safety assurance test.Thus, the aim of this study is to identify the potential acute toxicity of roots, stems, leaves,and flowers ethanol extracts of bamboo bamboo plants (Polygonum pulchrum Blume). Thisstudy used brine shrimp lethality test (BSLT) method. The total number of larvae used ineach concentrations in three times replications was 330 larvae. Each group was given consecutivelyroots, stems, leaves and flowers ethanol extracts of P. pulchrum Blume plants withvariation concentrations 12.5 ?g/ml, 25 ?g/ml, 50 ?g/ml, 100 ?g/ml, 125 ?g/ml, 250 ?g/ml, 500 ?g/ml, 1000 ?g/ml, 2000 ?g/ml, and 4000 ?g/ml. The number of dead larvae after24 hours treatment were calculated to obtain the mortality percentages and to determine theLC50 value, which were determined by probit analysis using MiniTab application version17.1.0. Ethanol extract of P. pulchrum roots and stems resulted LC50 values of 933.08 ?g/mland 919.58 ?g/ml, respectively. While the LC50 values of leaves and flowers extracts were2207.06 ?g/ml and 1081.90 ?g/ml, respectively. In conclusion, P. pulchrum Blume roots andstems were classified as toxic according to BLST method, while leaves and flowers were not.Keywords: acute toxicity, brine shrimp lethality test, Polygonum pulchrum Blume
Anti-Inflammatory Activity of Marine Sponge Aaptos sp. to the Plasma Interleukin-1β Level in Wistar Male Rats Fristiyohadi, Adryan; Wahyuni, Wahyuni; Kalimin, Wa OIL.; Permana, La OMJ.; Saripuddin, Saripuddin; Sahidin, Idin
Pharmacology and Clinical Pharmacy Research Vol 4, No 2
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (105.584 KB) | DOI: 10.15416/pcpr.v4i2.24269

Abstract

Inflammation is the response of the body to injury and infection characterized by swelling, heat, pain, and redness. This study aimed to investigate the anti-inflammatory effect of Aaptos sp. ethanolic extract to plasma interleukin (IL)-1? level of Wistar male rats. Aaptos sp. was macerated with 96% ethanol for 3 x 24 hours. Inflammation was induced with administration of 1% carrageenan intraplantarly. Animals were divided into 5 treatment groups, i.e., positive control  (diclofenac sodium 3598 ppm); Aaptos sp extract 50 ppm; Aaptos sp extract 100 ppm Aaptos sp extract 200 ppm; and negative control (0.5% Na CMC). After 1 hour, blood was collected and assayed using enzyme-linked immunosorbent assay (ELISA) kit. The results showed that plasma IL-1? levels of animals were decreased by Aaptos sp ethanolic extract. The administration of 50 ppm of extract showed no significant difference (p>0.05)  in IL-1? level in first and second hour measurement, but indicated a statistically significant decrease after three hour (p<0.05). The administration of 100 ppm of extract showed no significant difference (p>0.05) in every hour. Significant reduction was observed in the administration of 200 ppm of extract, but the elevation of IL-1? levels was also observed at third hour measurement. In conclusion, ethanolic extract of Aaptos sp. had anti-inflammatory activity and its effective dose was 50 ppm.