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All Journal Buletin Peternakan
Purnamasari, Listya
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EFFECTS OF METHIONINE-CYSTEINE AMINO ACID SUPPLEMENTATIONS IN THE AFLATOXIN B1 CONTAMINATED DIET ON BROILER PRODUCTION PERFORMANCE Purnamasari, Listya; Agus, Ali; Noviandi, Cuk Tri
Buletin Peternakan Vol 43, No 4 (2019): BULETIN PETERNAKAN VOL. 43 (4) NOVEMBER 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.86 KB) | DOI: 10.21059/buletinpeternak.v43i4.31150

Abstract

This research aimed to observe the interaction of methionine-cysteine amino acid supplementation to decrease the effect of aflatoxin B1 (AFB1) on diet against production performance of broiler chicken. A number of 240 mixed sex broiler chickens were treated in 9 treatments by factorial design 3 x 3 with methionine-cysteine amino acid (M+C) (75,100, dan 125%) factors and AFB1 levels (0, 200, dan 400 ppb). Variables observed were: Weight gain, feed consumption, and feed conversion ratio (FCR). The results showed that increased AFB1 content in diet from 0 to 400 ppb increased chicken body weight (P <0.05) in each age group. The high body weight was balanced with high feed consumption along with increased nutrient needs, mainly sulfuric amino acid (M+C) as the precursor of glutathione to eliminate toxic through conjugation reactions. The interaction effect was firstly occurred between M + C and AFB1 treatment (P <0.05). Meanwhile increased supplementation of M + C from 75 to 125% caused decreased feed consumption in each age group of chickens, but increased AFB1 levels further increased feed consumption (P<0.05). The interaction effect between the level of M + C and AFB1 contamination in diets on feed consumption were seen in 21-day-old chickens (P<0.05). FCR was also increased (P <0.05) with the reduction of M + C content in diet at 7 days old. The effect of AFB1 on diet and interaction between M + C and AFB1 on chicken FCR in this study was not significant in all age groups. It can be concluded from the current study that supplying methionine-cystine amino acid with 75, 100 and 125% in AFB1 contaminated diet of 0, 200 and 400 ppb improves the performance of broiler chicken production.
KAJIAN PRODUKSI AFLATOKSIN B1 KASAR DARI ISOLAT KAPANG ASPERGILLUS FLAVUS LOKAL PADA MEDIA JAGUNG DAN JAGUNG+KACANG TANAH purnamasari, listya; Agus, Ali; Noviandi, Cuk Tri
Buletin Peternakan Vol 40, No 2 (2016): BULETIN PETERNAKAN VOL. 40 (2) JUNI 2016
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (879.829 KB) | DOI: 10.21059/buletinpeternak.v40i2.9354

Abstract

Aflatoxin, which known as toxigenic compound, is a secondary metabolite produced by Aspergillus flavus and A. parasiticus. Aflatoxin is found in both food and feed stuffs, such as: corn and peanut. Aflatoxin standard is needed in every research of aflatoxin contamination. But it is rather difficult to get. It is imported, high costs, and take times. The aim of the research is to assess the potential local isolate of A. flavus to producing aflatoxin that can be used to be the alternative aflatoxin standard. Local mold of A. flavus FNCC 6122 and FNCC 6109 are got from PAU University of Gadjah Mada. Isolate was enriched on PDA medium for 5 days and move to corn medium of corn+peanut combine medium for 10-15 day at 25ºC. The variables was the content of AFB1 by ELISA test. The result is shown that isolat FNCC 6122 produced higher AFB1 than isolate FNCC 6109. The combine of corn and peanut medium stimulated FNCC 6122 to produce higher AFB1 and has potency to be the candidate of standard aflatoxin. In conclusion, the peanut addition on the medium would initiate increasing of the aflatoksin B1 level.