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All Journal Indonesian Journal of Cancer Chemoprevention
Muntafiah, Laeli
Indonesian Society for Cancer Chemoprevention
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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A Comparison of Antimetastatic Activity between Nerium indicum and Cinnamomum burmannii on 4T1 Cells Lestari, Beni; Muntafiah, Laeli; Walidah, Ziana; Jenie, Riris Istighfari
Indonesian Journal of Cancer Chemoprevention Vol 8, No 2 (2017)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (847.038 KB) | DOI: 10.14499/indonesianjcanchemoprev8iss2pp85-93

Abstract

Metastatic process becomes a major problem in advanced cancer cases. Natural compounds found in several plants in Indonesia have a potency to be developed as chemoterapeutic agent which are targeted to metastatic process. Jure leaves (Nerium indicum) which contain oleandrin and cinnamaldehyde in cinnamon bark (Cinnamomum burmannii) reported to have cytotoxic activity on several cancer cells, but their activities on metastasic process have never been explored. This research aims to reveal and to compare their anti-metastatic effect toward 4T1 breast cancer cells. The cytotoxicity of jure leaves extract (JLE) and cinnamon essential oil (CEO) was obtained by MTT assay. Metastatic process mainly on cell migration was examined by scratch wound healing assay while MMP-9 expression that described the invassion process was observed by gelatin zymography assay. Molecular interaction between their active compounds and MMP-9 receptor was predicted by molecular docking. The result showed that treatment with JLE and CEO inhibited the growth of 4T1 cells with IC50 value of 125 µg/mL and 2.5 µg/mL, respectively. In addition, JLE performed inhibitory effect of cell migration better than CEO. Meanwhile, both JLE and CEO decreased MMP-9 protein expression. Thus, JLE and CEO have potentials to be developed as an anti-metastatic agent and JLE could be more effective.Key words: Nerium indicum, Cinnamomum burmannii, anti-metastasis, scratch assay, gelatin zimography
Anti-Aging Activity Of Cucurbita moschata Ethanolic Extract Towards NIH3T3 Fibroblast Cells Induced By Doxorubicin Muntafiah, Laeli; Shabrina, Bani Adlina; Sulistyowati, Dwi; Asshagab, Murah Riski Novi; Jenie, Riris Istighfari
Indonesian Journal of Cancer Chemoprevention Vol 7, No 2 (2016)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (680.453 KB) | DOI: 10.14499/indonesianjcanchemoprev7iss2pp49-53

Abstract

Degenerative disease caused by decreasing organ function in old people has become the biggest death cause in the world. This aging process marked by senescence activity. An anti-aging agent from the medicinal plant has high potency to be developed, such as pumpkin seed (Cucurbita moschata) which contain tocopherol as antioxidant. The aim of this study is to investigate the anti-aging effect of pumpkin seed extract (PSE) on NIH 3T3 fibroblast normal cell induced by doxorubicin. This anti-aging effect was observed using MTT assay continued by SA- βgal (Senescence-Associated Beta-Galactosidase) activity detection test. The stability of  molecular interaction between tocopherol and doxorubicin to CYP 3A4 as the oxidase enzyme was conducted using molecular docking. Based on in vitro test, PSE is not cytotoxic to NIH 3T3. PSE at the dose of 100,200,400, and 800 μg/mL decreased % cell senescence by 2,77; 4,5; 6; and 18 times respectively. Meanwhile, in silico test indicated that tocopherol (-107,409) has a higher interaction to CYP 3A4 compared to doxorubicin (-70,52). Both compounds have a similar binding site in Leu 364; Phe 435; Pro 434; Cys 442; Ile 369; Thr 309; dan Ala 305. The overall result of this study showed that PSE has anti-aging effect by decreasing SA- βgal activity. This anti-aging activity possibly due to the interaction between tocopherol to CYP 3A4 based on molecular docking.Keywords: anti-aging, Cucurbita moschata, tocopherol, SA-βgal, molecular docking
Co-Authors Asshagab, Murah Riski Novi Beni Lestari, Beni Dwi Sulistyowati Riris Istighfari Jenie Shabrina, Bani Adlina Ziana Walidah, Ziana