Saputri, Dian
Indonesian Society for Cancer Chemoprevention

Published : 2 Documents
Articles

Found 2 Documents
Search

Reveal Cytotoxicity and Antigenotoxicity of Piper nigrum L. Ethanolic Extract and its Combination with Doxorubicin on CHO-K1 Cells Sari, Nur Fitra; Lestari, Beni; Saputri, Dian; Ahsani, Anisa Fauzia; Santoso, Ragil Anang; Sasmito, Ediati; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 8, No 3 (2017)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev8iss3pp110-119

Abstract

Black pepper (Piper nigrum L.), one of the most popular Indonesian spices has been reported to possess various therapeutic effects. The aim of this study is to evaluate the cytotoxicity and antigenotoxicity of black pepper ethanolic extract (BPE) and its combination with doxorubicin (Dox) on CHO-K1 cells. Based on thin layer chromatographyanalysis, BPE contained piperine.Under MTT assay, BPE showed cytotoxic effect with the IC50 value of 68 μg/mL and performed synergism in combination with Dox. In vitro micronucleus test using Giemsa staining revealed that BPE did not cause morphological changes qualitatively on CHO-K1 cells at concentration of 8.5 μg/mL, whereas using flow cytometry analysis showed that BPE could decrease the number of micronucleus (MN) formation induced by doxorubicin. In addition, BPE reduced the ROS level on the CHO-K1 cells which observed by reactive oxygen species (ROS) intracellular assay. The decrease in ROS level indicated that the antioxidant activity of BPE contribute to the antigenotoxicity. Furthermore, molecular docking performed that piperine interacted with DNA Topoisomerase II with docking score of -80.68. Overall,BPE performed cytotoxic effect in single treatment, increased the cytotoxicity and reduced the genotoxicity of doxorubicin. Thus, BPE has potential to be developed further as co-chemotherapeutic and antigenotoxic agent.Keywords: Cytotoxic, genotoxic, Piper nigrum L., CHO-K1, micronucleus
HEAVY METAL CONCENTRATIONS Cr, Cu, AND Zn OF SEAWEED Eucheuma cottonii IN MORO COSTAL WATERS KARIMUN DISTRICT RIAU ARCHIPELAGO PROVINCE Saputri, Dian; Amin, Bintal; Ikhwan, Yusni
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAK This research conducted on Juli 2018 to analyzed and compared the contain of Cr, Cu and Zn on E. cottonii from 3 station with different activity for each station. This research also to analyzed the consumption feasibility and the status of metal pollution using MPI (Metal Pollution Index). The result showed the average of concentrate of Cr 2,147 µg/g, Cu 0,9811 µg/g and Zn 9,3061 µg/g. The index value of metal pollution was 2,69 showed that the condition of water pollution at Moro, district of Karimun, Riau Island Province still at low condition. E. cottonii seaweed from this Moro's water thought that still safe for consumption as long as no more than 3,03 kg/week for Cr, 1020,83 kg/week for Cu, and 210,30 kg/week for Zn. Key words : Metal Pollution, Seaweed, E. cottonii, Riau Archipelago Province