Sari, Rita K
Jurnal Ilmu dan Teknologi Kayu Tropis

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Aktivitas Antibakteri Resin Pinus terhadap Staphylococcus aureus (Antimicrobial Activities of Pines Resin against Staphylococcus aureus) Sari, Rita K; Batubara, Irmanida; Tillah, Mardho; Tohir, Dudi
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 16, No 1 (2018): Jurnal Ilmu dan Teknologi Kayu Tropis
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Previous research showed that n-hexane extract of Pinus oocarpa resin was the most potential extract among extracts of Pinus resin from Indonesia as antibacterial against Staphylococcus aureus. The purpose of this study were to separate the active antibacterial component of n-hexane P. oocarpa resin extract and characterize the functional groups of its most active fractions. The component were separated by silica gel column chromatography and detected by antibacterial bioautography method. Fraction F1 is the most active from 11 fractions. Furthermore, F1 separated further by preparative thin layer chromatography and sephadex LH-20 column chromatography. Three fractions (F1.1.1-F1.1.3) retrieved from the separation by sephadex LH-20 column. Furthermore F1.1.2 had the minimum inhibitory concentration and minimum bactericidal concentration of 125 and 250 µg ml-1 respectively against S. aureus by microdilution method. Characterization of fraction F1.1.2 using Fourier transformed infrared (FTIR) spectrophotometer shows that this fraction allegedly containing diterpenoid compounds.
Bioactivity Test of Mindi Extractives (Melia azedarach Linn) Using Brine Shrimp Lethality Test Syafii, Wasrin; Sari, Rita K; Maemunah, Siti
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 12, No 1 (2014): Jurnal Ilmu dan Teknologi Kayu Tropis
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The aims of this research were to determine the yield of extracts from continuous extraction of heartwood, sapwood, inner bark, branch, and leave of Toona sinensis in n-hexane, ethyl acetate, and methanol solvents and their bioactivity using brine shrimp lethality test (BSLT) was evaluated. The results indicated that the yield of leave, inner bark, branch, heartwood, and sapwood extracts were 11.0, 6.7, 1.9, 1.6, and 1.6%, respectively. The highest yield of extracts was obtained from extraction in ethyl acetate (16.1%), followed by methanol (3.5%), and n-hexane (3.1%). The BSLT tests showed that the ethyl acetate extracts of all tree parts have higher bioactivities (LC50 1-52 μg ml-1) than the n-hexane extracts (LC50 40-181 μg ml-1), and the methanolic extracts (LC50 49-1375 μg ml-1). The ethyl acetate extract from inner bark has the highest bioactivity (LC50 μg ml-1). The qualitative analysis detect the ethyl acetate extract from inner bark containing saponins, alkaloids, phenolics, flavonoids, triterpenoids, steroids, and glycosides.Key words: Artemia salina, bioactivity, brine shrimp lethality test, Toona sinensis
Aktivitas Antioksidan dan Toksisitas Akut Zat Ekstraktif dari Residu Penyulingan Surian (Toona sinensis Roemor) Sari, Rita K; Melianti, Desi; Syafii, Wasrin; Agungpriyono, Dewi R
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 11, No 2 (2013): Jurnal Ilmu dan Teknologi Kayu Tropis
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The objective of this research was to determine the yield, antioxidant activity, acute toxicity, and phytochemical compound of extractives from left over distilling liquid and distillation residues of various part of surian (Toona sinensis). The extracts from the left over distilling liquid of leaves, heartwood, and sapwood were assigned as LL, HL, and SL, respectively, whereas LB, HB, and SB were respectively the extracts from leaves, heartwood, and sapwood of boiled solid residue. The yield of LL, LB, SL, HL, SB, and HB were 28.78, 10.18, 2.11, 1.74, 0.92, and 0.81% respectively. Based on antioxidant activity test, the LB, LL, and HL extracts were classified as very active with EC50 of 5.45, 5.70, and 5.91 mg ml-1 respectively. The SL was classified as active (EC50 32.36 mg ml-1), the SB and HB extracts were classified as inactive (EC50 > 100 mg ml-1). Based on acute toxicity test, LB is the best natural antioxidant because it was non toxic material (LD50 47752 mg kg-1 BW), while the LL and HL extracts were clasified as low toxicity with LD50 4518.56 and 1999.86 mg kg-1 BW (body weight). The LB extract contained phenol hydroquinone, flavonoids, and tannins (total phenolic content: 94 mg g-1 GAE).
Antiproliferative Activities of Anthocephalus cadamba Extracts on Human Breast Cancer and Cervical Adenocarcinoma Cell Lines Sari, Rita K; Armilasari, Devi; Nawawi, Deded S; Darmawan, Wayan; Mariya, Silmi
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 12, No 1 (2014): Jurnal Ilmu dan Teknologi Kayu Tropis
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The aim of this research was to evaluate the anticancer properties of methanolic extracts from inner bark and wood of jabon (Anthocephalus cadamba). The extracts were investigated in vitro bioassay for its possible antiproliferative activities on human MCF7 breast cancer cell line and HeLa cervical adenocarcinoma cell lines. The cell viability were assessed using microculture tetrazolium technique (MTT) colorimetric assay. The results showed that inner bark extract exhibited higher antiproliferative activity on MCF7 cancer cell line (IC50 91 μg ml-1) than wood extract (IC50 312 μg ml-1). But, antiproliferative activity of inner bark extract on HeLa cell lines was higher (IC50 5 μg ml-1). The inner bark extract is potential to be developed as anti cancer agent in cervical adenocarcinoma cancer therapy because moresecure against Vero normal cells (IC50 288 μg ml-1). Whereas compounds such as phenolic and fatty acid contribute to high antiproliferative activities of inner bark extract. The qualitative analysis detect the extracts containing flavonoids, triterpenoids, saphonin which are thought to contribute to the high antiproliferative activities of this extract.Keywords : Anthocephalus cadamba, antiproliferative activity, human MCF7 breast cancer cell line, HeLa cervical adenocarcinoma cell lines, Vero normal cell lines
Aktivitas Antioksidan dan Tabir Surya Ekstrak Daun Gyrinops verstegii (Antioxidant activity and Sunscreen of Gyrinops versteegii Leaf Extract) Wahyuningrum, Maeda; Sari, Rita K; Rafi, Mohamad
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 16, No 2 (2018): Jurnal Ilmu dan Teknologi Kayu Tropis
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The aim of this study was to determine the yield and phytochemicals, antioxidant activity, and sunscreen of the Gyrinops versteegii leaf extracts. The leaf simplicia was extracted using soxhletation method with multilevel polarities of solvent (n-hexane, ethyl acetate, and metanol). The analysis of phytochemical extracts has been carried out the qualitatively and quantitatively. The antioxidant activity testing was performed in vitro through the effective concentration (EC50) extract in capturing DPPH radicals. Sunscreen activity has been done through testing sun protection factor (SPF). The result showed that the yield of n-hexane extract, ethyl acetate, and methanol extracts were 7.83, 5.46, and 6.77% respectively. The phytochemical analysis showed that the ethyl acetate and methanol extracts were strongly detected containing antioxidant compounds such as p-hydroquinone, flavonoid, and tannins with the total phenol of the ethyl acetate and methanol extracts were 3.40 and 4.27% respectively. The n-hexane extract detected contains weakly the antioxidant compounds with the total phenol was 0.45%. The methanol extract is the highest antioxidant activity (EC50 14.46 μg ml-1) and has ultra sunscreen activity (SPF>15).Keywords: antioxidant, extractive, G. versteegii, sunscreen
Characterization of Cat’s Eye Dammar for Revision of Indonesia National Standard Sari, Rita K; Wistara, Nyoman J; Wijayanto, Arif; Waluyo, Totok K
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 11, No 1 (2013): Jurnal Ilmu dan Teknologi Kayu Tropis
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The present works was aimed to examine the concordance between trader quality classification and related SNI classification on dammar resin. The quality of six quality classes of dammar resin by trader classification was evaluated based on SNI 2900-1-2012 on visual classification and SNI 01-2900-1999 on physical and chemical characteristics, and then the resulting data were analyzed with ANOVA. The quality concordance of dammar resin based on trader classification with these of SNI for visual appearance and the size of agglomeration was 50 and 75-94%, respectively, and these of insolubility in toluene, melting point, ash content, and acid number were 61, 72, 11, and 100%, respectively. The characteristics of dammar resin for the a, b, and c quality classification were the same for the Krui traders, BKG Ltd., and WGM Ltd. However, they were different for the lower quality classification of d, e, and powder. It was also found the existence of dammar resin with characteristic values beyond the range of these established by SNI. SNI should not classify dammar resin quality based on visual appearance, but merely based on its physical and chemical characteristics. Physical and chemical characteristics values required by SNI should be revised.Key words: cat’s eye dammar, Indonesia National Standard, physical and chemical characteristics, quality classification, Shorea javanica
Anticancer Activity and Chemical Compounds of Suren Heartwood Extract (Toona sureni) Sari, Rita K; Syafii, Wasrin; Achmadi, Suminar A; Hanafi, Muhammad; Laksana, Yanotama T
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 10, No 1 (2012): Jurnal Ilmu dan Teknologi Kayu Tropis
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The aims of this research were to determine the yield extracts from continuous extraction of suren heatwood (Toona sureni) in n-hexane, ethyl acetate and methanol solvents, to evaluate this extracts using in vitro anticancer tests (antioxidant, brine shrimp lethality test /BSLT, antiproliferative effects to HeLa servical cancer cell lines, Raji limphoma cancer cell lines, and Vero normal cell lines), and to analyze the best extract based on the anticancer activities. This experiment showed that the yield of methanolic, ethyl acetate, and n-hexane extracts were 0.43%, 0.25%, and 0.18% respectively. The methanolic and ethyl acetate extracts had the high antioxidant activities with EC50 51 and 68 μg ml-1 respectively. Based on BSLT, the ethyl acetate extract was the most active extract (LC50 40 μg ml-1), it followed by the methanolic extract (LC50 116 68 μg ml-1 ), and the n-hexane extract (LC50 161 68 μg ml-1). Further testing showed that the ethyl acetate extract had high antiproliferative effects to Raji (IC50 31 μg ml-1) and HeLa (IC50 65 μg ml-1), but it was more secure against Vero cell lines (IC50 105 μg ml-1). Whereas compounds such as catechol, linalool, and sitosterol contributed to the high anticancer activities of this ethyl acetate extract.Key words: anticancer activity, HeLa cell lines, Raji cell lines, Toona sureni, Vero cell lines
Aktivitas Larvasida Aedes aegypti Ekstrak Kayu Jati Hasil Ekstraksi Hidrotropi (Larvicidal Activity Against Aedes aegypti of Teak Wood Extracts from Hydrotropic Extraction) Sari, Rita K; Iskandar, Idris N; Priambodo, S; Pramesti, Regita; Amalia, Livia T
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 15, No 2 (2017): Jurnal Ilmu dan Teknologi Kayu Tropis
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This study aimed to determine the yield and chemical content of teak extracts from hydrotropic extraction and larvalidal activity test against Aedes aegypti. The teak wood powder was extracted with urea (U) and natirum acetate (NA) solutions at 1M and 2M with physical treatment of magnetic stirrer (MS) and microwave (MS). The composition of chemical compound of extracts were determined by GC-MS. The larvicidal activity test were conducted on insted larvae of A. aegypti mosquito III. The results showed that the yield of NA extracts were higher (1.08-2.31%) than U extracts (0.72-1.68%). The increased of solvent concentration increased the extract yield. The physical treatment of MS produced a higher extract than MW. The NA extracts were dominated by acetic acid (16.53-40.21%), whereas the U extracts contained various dominant compounds. Both NA and U extracts contained tectoqinon, eugenol, farnesol, o-methoxyl benzene alcohol, methoxydeugenol, 3-penten-2-one, and tetracosahexaene with different levels. The U extracts were detected containing tectoqinon (0.66-1.79%), while the Na extract which was extracted with 1M NA and got MW treatment was detected contain tectoqinon (0.42%). The NA extracts were classified as active as larvacidal to A. aegypti, while U extracts were classified as inactive as larvacidal to A. aegypti.Keywords: Aedes aegypti, hydrotropic extraction, larvacidal, teak wood extract
Eksplorasi Tumbuhan Obat Hutan Berkhasiat Inhibitor HMG-KoA Reduktase (Exploration of Medicinal Forest Plants with HMG-CoA Reductase Inhibitory Activity) Sulistiyani, Sulistiyani; Sari, Rita K; Triwahyuni, Wulan
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 15, No 2 (2017): Jurnal Ilmu dan Teknologi Kayu Tropis
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Five medicinal forest plants are potential candidates to be developed as cholesterol-reducing herbal product. The objective of this research is to explore the potency of the leaf of pulai ((Alstonia scholaris, R.Br), jabon merah (Antocephalus macrophyllus), jati belanda (Guazuma ulmifolia), mindi (Melia azedarach), and surian (Toona sinensis) based on their main secondary metabolic contents and to study their activity as HMG-CoA reductase inhibitor, an enzyme key in the regulation of cholesterol biosynthesis. Dried leaves materials were prepared from the each plant by standard procedure and their pythochemicals contents were determined qualitatively. Subsequently, flavonoid fractions from the leaves of all five plants were prepared, and alkaloid fraction was prepared from the leaf of pulai only. The flavonoid content was determined using quercetin as standard. The effect of each samples on the HMG-CoA reductase activity were analyzed using HMGR in vitro assay kit. At concentration of 20 ppm jati belanda leaf extract, however, showed the highest inhibitory activity which was similar to quercetin (5 ppm) as well as pravastatin control inhibitor. Further analysis showed that extracts from leaves of all plants tested were shown to inhibit the HMG-CoA reductase with IC50 values ranging from 7.53 to 52.62 ppm.Keywords: HMG-KoA reductase inhibitor, in vitro assay, medicinal forest plant exploration
Antioxidant and Tyrosinase Inhibitor Activities of Methanol Extracts of Acacia mangium Sari, Rita K; Utami, Rahmi; Batubara, Irmanida; Carolina, Anne; Febriany, Salina
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 13, No 1 (2015): Jurnal Ilmu dan Teknologi Kayu Tropis
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Active compounds utilization of its extractives for cosmetic products increase the value added of mangium tree. The purpose of this study was to determine the antioxidant and an inhibitor tirosinase activities of methanol extract of the various parts of mangium tree. Phytochemical properties of the best extract were also anlayzed. Extraction was conducted by soxhletation in methanol for 12 hours. The antioxidant and tyrosinase inhibitory activities of the extracts were tested in vitro to radical of 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging and inhibition to tyrosinase enzyme. The results showed that leave resulted in the highest methanol ectract followed succesively by bark, heartwood, and sapwood the value of 10.7; 4.4; 2.5; and 0.9%, respectively. The highest antioxidant activity was bark extract and followed by leave, heartwood, and sapwood extracts with EC50 values respectively of 8.3; 26.7; 66.9; and 137.9 ppm. Only bark extract which classified as an active tyrosinase inhibitor with IC50 value of 257.8 ppm in the difenolase reaction. The IC50 value of the positive control (kojic acid) was 116.7 ppm. The other extracts relatively inactive as a tyrosinase inhibitor because their IC50 values > 1000 ppm. The qualitative analysis detect the methanol extract of mangium bark as the best extract containing phenolic compounds (phenol hirokinon, flavonoids, and tannins) and alkaloids which were thought to contribute to the high antioxidant and tyrosinase inhibitor activities.Keywords: Acacia mangium, antioxidant, extracts, tyrosinase inhibitor